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| 抑癌候选基因NDRG2在甲状腺癌组织中的表达及其意义 | |
| 引用本文： | 赵华栋,付强,张健,鲁建国,何显力,南菁,杨媛,药立波.抑癌候选基因NDRG2在甲状腺癌组织中的表达及其意义[J].现代生物医学进展,2009,9(18). |
| 作者姓名： | 赵华栋 付强 张健 鲁建国 何显力 南菁 杨媛 药立波 |
| 作者单位： | 1. 第四军医大学唐都医院普通外科,陕西,西安,710038 2. 第四军医大学唐都医院泌尿外科,陕西,西安,710038 3. 第四军医大学生化教研室,陕西,西安,710032;第四军医大学唐都医院普通外科,陕西,西安,710038 4. 第四军医大学生化教研室,陕西,西安,710032 |
| 基金项目： | 国家高技术研究发展计划(863计划),国家自然科学基金 |
| 摘 要： | 目的:研究抑癌候选基因NDRG2在人类甲状腺癌组织及其癌旁组织中的表达情况.方法:收集30例甲状腺癌组织及其癌旁组织,提取总RNA,应用半定量RT-PCR方法检测NDRG2 mRNA的表达水平.分别提取30例组织的总蛋白,应用蛋白印迹技术检测其NDRG2的蛋白表达水平.结果:RT-PCR结果显示,30例甲状腺癌组织中,有25例NDRG2的mRNA水平明显降低,蛋白印迹结果显示,30例甲状腺癌组织中发现25例NDRG2的蛋白水平明显下降,与RT-PCR检测结果一致.结论:NDRG2在甲状腺癌组织中呈低表达,提示其可能对甲状腺癌的发生或发展有重要作用影响. |
| 关 键 词： | NDRG2 甲状腺癌 逆转录聚合酶链反应 蛋白印迹法 |
Expression analysis of tumor suppressor gene NDRG2 in thyroid cancer tissues |
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| ZHAO Hua-dong,FU Qiang,ZHANG Jian,LU Jian-guo,HE Xian-li,NAN Jing,YANG Yuan,YAO Li-bo.Expression analysis of tumor suppressor gene NDRG2 in thyroid cancer tissues[J].Progress in Modern Biomedicine,2009,9(18). | |
| Authors: | ZHAO Hua-dong FU Qiang ZHANG Jian LU Jian-guo HE Xian-li NAN Jing YANG Yuan YAO Li-bo |
| Institution: | ZHAO Hua-dong1,FU Qiang2,ZHANG Jian3,1,LU Jian-guo1,HE Xian-li1,NAN Jing1,YANG Yuan1,YAO Li-bo3 (1 Department of General Surgery,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China,2 Department of Urology Surgery,3 Department of Biochemistry& Molecular Biology. School of Basic Medicine,Xi'an 710032,China) |
| Abstract: | Objective: To investigate the expression of candidate tumor suppressor gene NDRG2 in human thyroid cancer tissues. Methods: Thirty samples of thyroid cancer tissues with matched normal tissues were collected. The NDRG2 mRNA level was detected by RT-PCR and the NDRG2 protein level was analyzed by western blot. Results: The results of RT-PCR and western blot detection indicated that thirty samples of the twenty-five thyroid cancer tissues had low NDRG2 mRNA level and low protein level. Conclusion: NDRG2 has l... |
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