| 用AFLP标记快速构建遗传连锁图谱并定位一个新基因tms5 |
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| 引用本文： | 贾建航,李传友,邓启云,王斌.用AFLP标记快速构建遗传连锁图谱并定位一个新基因tms5[J].Acta Botanica Sinica,2003,45(5):614-620. |
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| 作者姓名： | 贾建航 李传友 邓启云 王斌 |
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| 作者单位： | 中国科学院遗传与发育生物学研究所 北京100101
(贾建航，李传友，邓启云)，中国科学院遗传与发育生物学研究所 北京100101(王斌) |
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| 基金项目： | 国家转基因植物研究与产业化专项 (J99_A_0 0 2 )，国家重点基础研究发展规划项目 (2 0 0 1CB10 880 2 )。~~ |
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| 摘 要： | 报导了一个分子标记连锁图的快速构建方法。通过对水稻(Oryza sativa L．)“安农S-1”和“南京11”的F2分离群体的AFLP分析找到了142个AFLP标记，用这142个AFLP标记以及已定位的25个SSR标记和5个RFIP标记构建了水稻12个染色体的分子标记连锁图，该图覆盖水稻基因组的1537．4cM，相邻标记间的平均间距为9．0cM，这是在国内建立的第一张AFLP标记连锁图。在建立连锁图谱的同时把一个新基因tms5(水稻温敏核不育基因)定位在第2染色体上。
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| 关 键 词： | 遗传连锁图 水稻 温敏核不育基因 AFLP |
Rapid Constructing a Genetic Linkage Map by AFLP Technique and Mapping a New Gene tms5 |
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| Authors: | JIA Jian_Hang LI Chuan_You DENG Qi_Yun WANG Bin |
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| Institution: | JIA Jian_Hang,LI Chuan_You,DENG Qi_Yun,WANG Bin * |
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| Abstract: | In this study, we reported the repaid construction of a molecular marker linkage map of rice ( Oryza sativa L.). An F 2 population from the cross between Annong S_1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1 537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was tigged and mapped onto chromosome 2 during the development of the linkage map. |
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| Keywords: | AFLP rapid constructing genetic linkage map rice thermosensitive genic male sterile gene tms 5 |
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