| 转基因抗草甘膦油菜的检测方法 |
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| 引用本文： | 李闻娟,张建平,王少宏,孙佳,党占海,赵利,赵玮,王利民,党照.转基因抗草甘膦油菜的检测方法[J].生物技术通讯,2012,23(2):242-244. |
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| 作者姓名： | 李闻娟 张建平 王少宏 孙佳 党占海 赵利 赵玮 王利民 党照 |
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| 作者单位： | 1. 甘肃省农业科学院作物研究所,甘肃兰州,730070
2. 甘肃农业大学,甘肃兰州,730070 |
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| 摘 要： | 目的:建立检测田间转基因抗草甘膦油菜的方法。方法:用CTAB法提取DNA,经PCR分别扩增内参基因BnACCg8,及抗草甘膦外源基因CP4-EPSPS、FMV 35S启动子和E9 3’终止子等4种基因的片段,以琼脂糖凝胶电泳对扩增产物进行分析比对。结果:抗草甘膦油菜中分别扩增出与外源基因FMV 35S启动子、E9 3’终止子和CP4-EPSPS大小一致的片段。结论:该方法能有效地用于转基因抗草甘膦油菜的检测。
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| 关 键 词： | 抗草甘膦 转基因 油菜 PCR检测 |
A Method for Detection of Glyphosate Resistant Transgenic Rape |
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| LI Wen-Juan
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ZHANG Jian-Ping
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WANG Shao-Hong
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SUN Jia
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DANG Zhan-Hai
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ZHAO Li
,
ZHAO Wei
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WANG Li-Min
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DANG Zhao.A Method for Detection of Glyphosate Resistant Transgenic Rape[J].Letters in Biotechnology,2012,23(2):242-244. |
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| Authors: | LI Wen-Juan ZHANG Jian-Ping WANG Shao-Hong SUN Jia DANG Zhan-Hai ZHAO Li ZHAO Wei WANG Li-Min DANG Zhao |
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| Institution: | 1. Crops Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070; 2. Gansu Agricultural University, Lanzhou 730070; China) |
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| Abstract: | Objective： To develop a method for detection of transgenic glyphosate resistant rape. Methods： DNA of glyphosate resistant rape was extracted by the method of CTAB, then reference gene BnACCg8, and three exoge nous genes of FMV 35S promoter, E9 3 terminator and CP4EPSPS were amplified by PCR, and the amplified products were detected and analyzed on agar sugar gel electrophoresis. Results： There were three fragments which as same size as three exogenous genes of FMV 35S promoter, E9 3 terminator and the CP4EPSPS respectively in glyphosate resistant rape. Conclusion： This method can be applied in determination of transgenie glyphosate re sistant rape. |
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| Keywords: | glyphosate genetically modified rapeseed PCR detection |
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