Genetic variation in Armillaria mellea subsp. nipponica estimated using IGS-RFLP and AFLP analyses |
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| Authors: | Kazuhisa Terashima Joo Young Cha Eiji Nagasawa Kiyoshi Miura |
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| Institution: | (1) Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan;(2) Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Japan;(3) The Tottori Mycological Institute, The Japan Kinoko Research Centre Foundation, Tottori, Japan;(4) Present address: The Tottori Mycological Institute, The Japan Kinoko Research Centre Foundation, Tottori 689-1125, Japan;(5) Present address: 1-6-16-18 Ishiyama, Sapporo 005-0841, Japan |
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| Abstract: | In this study, genetic variation of Armillaria mellea subsp. nipponica was estimated using intergenic spacer-restriction fragment length polymorphism (IGS-RFLP) and amplified fragment length polymorphism
(AFLP) analyses. Four IGS-RFLP phenotypes were produced, of which two have never been reported. AFLP analysis suggested that
the 11 isolates used could be divided into five subgroups, and the isolates within the same subgroup were distributed throughout
a relatively large area in Japan. A parental isolate and its offspring (single-spore isolates) showed an almost identical
AFLP profile to each other. These results suggest that the large distribution of the isolates within the same subgroup were
established via the basidiospore from a common parental strain.
Contribution no. 378 of The Tottori Mycological Institute |
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| Keywords: | AFLP Armillaria mellea subsp nipponica Homothallic sexuality IGS-RFLP |
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