Glutathione S-transferase activities in rat and mouse sperm and human semen |
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Authors: | Hasan Mukhtar Insu P Lee John R Bend |
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Institution: | Laboratory of Pharmacology, National Institute of Environmental Health Sciences, P. O. Box 12233, Research Triangle Park, NC 27709 USA |
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Abstract: | Glutathione -transferase activity was found in sperm of the rat and and C57 mice. In rat sperm activities with benzo(a)pyrene 4,5-oxide, styrene 7,8-oxide, and 1-chloro-2,4-dinitrobenzene were 0.88, 1.07, and 26.1 nmoles/min/mg protein, respectively. Δ5-3-Ketosteroid isomerase activity of rat sperm was 4.9 nmoles/min/mg protein. These specific glutathione -transferase and Δ5-3-ketosteroid isomerase activities in sperm represent 0.4–4.1% of rat liver cytosol values. Human semen also contained significant glutathione -transferase activity. It is postulated that these enzymes could function in the metabolism and detoxification of certain electrophilic xenobiotics, if present in sperm. |
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Keywords: | SO styrene 7 8-oxide 4 5-BPO benzo(a)pyrene 4 5-oxide CDNB 1-chloro-2 4-dinitrobenzene HEPES N-2-hydroxypiperazine-N'-2-ethane sulfonic acid PBS (0 01 M sodium phosphate 0 145 M sodium chloride pH 7 2) CTAB cetyltrimethylammonium bromide |
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