A procedure for axenic isolation of the marine microalga Isochrysis galbana from heavily contaminated mass cultures |
| |
Authors: | Ji-Young Cho Jae-Suk Choi In-Soo Kong Soo-Il Park Russell G Kerr Yong-Ki Hong |
| |
Institution: | (1) Department of Biotechnology, Pukyong National University, Namku, 608-737, Korea;(2) Department of Aquatic Life Medicine, Pukyong National University, Namku, 608-737, Korea;(3) Department of Chemistry and Biochemistry, Center for Molecular Biology and Biotechnology, Florida Atlantic University, Boca Raton, 33431, USA |
| |
Abstract: | Isochrysis galbana, one of the most widely usedmarine microalgae in the rearing of finfish and shellfish larvae, is masscultured frequently in outdoor tanks. Under prolonged and repeated culture,severe contamination occurs. Axenic isolation of I.galbanafrom such cultures was best achieved by using a ternary procedure involvingpercoll-gradient centrifugation, treatment with antibiotics, and growth on agarmedium. Protozoa and other algae were removed most effectively by isolation ofI. galbana at the 30–40% density layer on apercoll-gradient. Removal of bacteria was accomplished using a mixture of 5antibiotics (250 g mL–1 ampicillin, 50g mL–1 gentamycin, 100 gmL–1 kanamycin, 500 gmL–1 neomycin, 50 gmL–1 streptomycin). Axenic colonies were isolated fromasolid medium prepared from 1% purified agar. The ternary procedure isconsideredapplicable to the isolation of other axenic single-celled microalgae fromheavily contaminated cultures. |
| |
Keywords: | Antibiotics Axenic isolation Gelling agent Isochrysis galbana Microalgae Percoll |
本文献已被 SpringerLink 等数据库收录! |
|