葛根素对体外培养的人老年女性骨质疏松症成骨细胞分化的初步研究

目的:观察葛根素对体外培养老年女性骨质疏松症骨折患者体外分离培养的成骨细胞分化的影响。方法:采集老年女性骨质疏松症股骨颈骨折行人工股骨头置换术中取下的股骨颈松质骨,采用骨组织块法原代培养成骨细胞。观察不同浓度(0、0.01、0.1、1μmol/L)葛根素对成骨细胞表达碱性磷酸酶(alkaline phosphatase,ALP)、骨钙素(osteocalcin,OC)的影响,并比较各组成骨细胞钙化能力的大小。结果:0.01~1.00μmol/L的葛根素随着浓度增加,成骨细胞表达ALP、OC活性呈增强趋势,各浓度组两两比较差异均有统计学意义(P0.05);随葛根素浓度增高,成骨细胞钙化结节数量也明显增加,组间比较差异有统计学意义(P0.05)。结论:葛根素呈浓度依赖性促进成骨细胞表达ALP、OC并能提高成骨细胞的钙化能力。

Influence of Puerarin on the Differentiation of Osteoblasts in Patients with Senile Women with Osteoporosis

Objective:To observe the influence of puerarin on the differentiation of osteoblasts in patients with senile women with osteoporosis in vitro.Methods:The cancellous bone of elderly women with osteoporosis artificial femoral head replacement patients was collected. The osteoblasts were cultured by bone tissue block method.To observe ALP and OC expressed by osteoblasts with different concentrations (0, 0.01, 0.1, 1 mol/L) puerarin.Results:With the increase of the puerarin concentration(0.01~1 mol/L), the expression of ALP and OC in osteoblasts were increased, the concentration of two groups were statistically significant (P<0.05). With the increase of the concentration of puerarin, osteoblast numbers of calcified nodules increased, there were significant differences between the groups (P<0.05).Conclusion:0.01, 0.1, 1 mol/L puerarin dose dependently stimulated osteoblasts express ALP and OC, and can improve the calcification capacity of osteoblasts.