A genetic map of melon (Cucumis melo L.) based on amplified fragment length polymorphism (AFLP) markers |
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Authors: | Y-H Wang C E Thomas R A Dean |
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Institution: | (1) Department of Plant Pathology and Physiology, 120 Long Hall, Clemson University, Clemson, SC 29634-0377, USA Fax:+1-864-656-0274 e-mail: rdean@clemson.edu, US;(2) US Vegetable Laboratory, USDA-ARS, 2875 Savannah Highway, Charleston, SC 29414, USA, US |
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Abstract: | Genetic maps facilitate the study of genome structure and evolution, and the identification of monogenic traits or Mendelian
components of quantitative traits. We evaluated 228 RAPD, microsatellite and AFLP markers for linkage analysis in melon (Cucumis melo L.) varieties MR-1 (resistant to Fusarium wilt, powdery and downy mildews) and Ananas Yokneum (AY; susceptible to these diseases) and constructed a detailed genetic
map. The mapping population consisted of 66 backcross progenies derived from AY×(MR-1×AY). Despite a relatively low level
of polymorphism in the species, AFLP markers were found to be more efficient in mapping the melon genome than RAPD or microsatellite
markers. The map contains 197 AFLPs, six RAPDs and one microsatellite marker assigned to 14 major and six minor linkage groups,
and covers 1942 cM with the average distance between adjacent markers of approximately 10 cM. The maximum distance allowed
between markers is 27.5 cM. About 11% of the intervals (20 out of 173) are over 20 cM (but less than 27.5 cM). The map has
immediate utility for identifying markers linked to disease resistance genes that are suitable for marker-assisted breeding.
The use of microsatellite markers for integration with other maps is also discussed.
Received: 12 March 1997 / Accepted: 20 May 1997 |
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Keywords: | Key words Melon Cucumis melo L Genetic mapping AFLP RAPD Microsatellite DNA markers |
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