| 从cDNA文库中筛选分析阳性克隆的简便方法 |
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| 引用本文： | 周兆斓,朱祯,刘春明,肖桂芳,李向辉.从cDNA文库中筛选分析阳性克隆的简便方法[J].生物化学与生物物理进展,1995,22(6):565-566. |
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| 作者姓名： | 周兆斓 朱祯 刘春明 肖桂芳 李向辉 |
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| 作者单位： | 中国科学院遗传研究所;中国科学院遗传研究所;中国科学院遗传研究所;中国科学院遗传研究所;中国科学院遗传研究所 |
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| 基金项目： | 总理基金及“863”计划基金资助. |
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| 摘 要： | cDNA文库中阳性克隆的传统筛选分析法既费时又费力.利用微波炉加热的方法,简化了原位杂交中噬菌斑裂解、DNA变性与固定的程序;进一步运用PCR扩增技术,特异扩增克隆载体中插入的cDNA片段,加快了阳性克隆分析的进程.
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| 关 键 词： | cDNA文库 杂交筛选 阳性克隆分析 |
| 收稿时间： | 1994/12/17 0:00:00 |
| 修稿时间： | 1995/2/21 0:00:00 |
Introduce a Rapid Method to Screening and Analysis of a cDNA Library |
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| Zhou Zhaolan,Zhu Zhen,Liu Chunming,Xiao Guifang and Li Xianghui.Introduce a Rapid Method to Screening and Analysis of a cDNA Library[J].Progress In Biochemistry and Biophysics,1995,22(6):565-566. |
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| Authors: | Zhou Zhaolan Zhu Zhen Liu Chunming Xiao Guifang and Li Xianghui |
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| Institution: | Institute of Genetics; Chinese Academy of Sciences;Institute of Genetics; Chinese Academy of Sciences;Institute of Genetics; Chinese Academy of Sciences;Institute of Genetics; Chinese Academy of Sciences;Institute of Genetics; Chinese Academy of Sciences |
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| Abstract: | In situ hybridization is a necessary and hard work for screening cDNA library.Heated by microwave oven,bacteriophage lysis,DNA denaturation and fixation of DNA to filter occur in a single step;On the other hand,it is a really rapid procedure for analysis of the cDNA inserts of positive clones via polymerase chain reaction (PCR) amplification. |
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| Keywords: | cDNA library in situ hybridization analysis of positive clones |
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