清醒小鼠严重颅脑闭合性撞击伤模型的建立

目的-建立一种清醒小鼠严重颅脑闭合性撞击伤模型, 模拟交通事故和战伤中的清醒致伤过程, 为相关研究创造新的动物模型基础。方法-将雄性KM小鼠头戴钢盔后清醒固定, 用BIM III型小型多功能动物撞击机击打, 致伤后2 h、8 h、24 h、48 h、72 h、120 h、1 w、2 w进行神经行为学评分、运动功能评分、死亡小鼠脑组织和肺组织水含量、死亡率、病理切片和电镜观察。结果-致伤后 48 h内致伤组运动功能评分、神经行为学评分明显低于对照组 (P<0 05); 致伤后死亡率高, 各组死亡小鼠脑组织水含量无明显差异, 但致伤组肺组织水含量明显高于对照组 (P <0 05); 病理切片和电镜检查显示, 致伤后脑组织细胞肿胀、坏死。结论-基本成功建立了模拟交通事故和战伤的清醒小鼠严重颅脑闭合性撞击伤模型。

Establishment of severe traumatic brain closed injury animal model in nonanesthetic mice

Objective To establish an animal model of severe traumatic brain closed injury (closed TBI) in traffic or war in mice. Methods Severe closed TBI in mice was performed by a BIM-III biomechanical machine with (non-anesthesia). At the time points of 2h, 8h, 24h, 48h, 72h, 120h, 1w, 2w after injury, the neurobehavior of mice was measured by neurological examination and motor functional test. Besides, cerebral and lung fluid content, mortality rate, pathologic and ultrastructure picture were analyzed. Results 48h after TBI, the neurobehavioral and motor function scores decreased significantly than that in control group (P<0.05), the mortality rate was high, there were no significant differences in lung fluid content among dead mice in different groups, but the fluid content in injury group was significantly higher than that in control group(P<0.05), and brain injury such as cells tumefaction, necrosis were confirmed by pathologic and ultrastructure technique. Conclusion The imitation would be a valuable animal model for experimental investigation of severe TBI in accident of traffic or war.