绿豆种子休眠性和百粒重的QTLs和互作分析

利用绿豆Berken/ACC41重组自交系在北京种植得到的121个F10家系和79个RFLP分子标记,采用改进复合区间作图法对绿豆种子休眠性和百粒重进行数量性状基因定位及上位性互作分析.检测到与发芽势有关的QTL 3个,与发芽率有关的QTL 4个,分别位于第1、11连锁群,解释表型变异的8.17%～12.14%和4.34%～12.69%.检测到与百粒重有关的QTL 5个,分别位于第2、8、9、11连锁群,解释表型变异的4.58%～10.36%.增加发芽率和百粒重的基因效应均来自母本Berken.分别检测到发芽势、发芽率和百粒重的加性×加性上位性互作8、9、9对,对这3个性状的总表型贡献率分别达到66.58%、47.91%、39.90%.本文初步分析了休眠性和百粒重的关系,并与前人的研究结果作了比较,旨在通过分子标记辅助选择,培育适度休眠的优良绿豆品种,进而解决绿豆收获前的荚上子粒发芽问题.

QTL Mapping and Interaction Analysis for Seed Dormancy and Seed Weight of Mungbean

Seed dormancy and seed weight were analyzed by using a Recombinant-inbred-lines (RIL) population from the cross between a no dormant parent Berken and a highly dormant wild parent ACC41. The RIL population were planted in Beijing and evaluated for their geminating energy, germinating percentage and 100-seed weight in the laboratory of CAAS. Software ICiMappingV1.3 was applied to detect the additive and epistatic QTLs. As a result, three QTLs on group 1 and 11 for geminating energy, four QTLs on group 1 and 11 for germinating percentage, five QTLs for 100-seed weight on group 2,8,9,11 were detected, explained 8.17%-12.14%, 4.34%-12.69% and 4.58%-10.36% of the phenotypic variations respectively. 26 pairs of interaction were detected, contributed 66.58%, 47.91%, 39.90% of the phenotypic variations respectively. In order to breed weather-tolerant mungbean varieties with large seeds, the relationship between seed dormancy and seed weight was analyzed and compared with previous research.