贵州苏铁种子钙形态特性分析

通过测定贵州苏铁新鲜成熟种子中不同组织中总钙和不同形态类型钙物质含量,探索贵州苏铁种子发育过程中对钙的储存形式和分布规律。结果表明贵州苏铁新鲜成熟种子中总钙含量为外种皮>中种皮>胚;钙主要集中在外种皮并且主要以HCl钙形态存在,其含量高达470.8 mg/kg,占外种皮钙形态的56.3%,以及组织和整个种子的24.6%和11.4%,HCl钙形态在种子胚的发育过程中主要起到源和库的作用;中果皮和胚主要积累的是水溶性的有机钙,含量分别为70.2 mg/kg和70.6 mg/kg,对参与种子的结构和胚的发育起到积极作用。另外,贵州苏铁种子中可溶性蛋白质和可溶性糖含量与外种皮高钙含量和高钙形态含量密切相关,而大分子物质的积累对促进贵州苏铁适应高钙质环境具有积极意义。

Analysis on the different characteristic of calcium in the seed of Cycas guizhouensis

The total calcium and the content of characteristic of calcium variation in different tissues of fresh ripe seeds of Cycas guizhouensis are determined,then the store mechanism and the distribute regularity of calcium in C. guizhouensis seeds during its growth are explored.The results showed that the total calcium in fresh ripe seeds of C. guizhouensis were decreased with the order;episperm>mesocarp>embryo;the mainly variation of calcium HCl-calcium in episperm,which are 470.8 mg/kg,accounted for 56.3%in emisperm,24.6%in tissue,and 11.4%in entire seed,respectively,it's play important roles of fountain and warehouse during the growth of embryo;the mainly calcium variation in mesocarp and embryo was organic soluble calcium,which are 70.2 mg/kg in mesocarp and 70.6 mg/ kg in embryo,and the organic soluble calcium have position function in building the structure of seeds and in the growth of embryo.Additionally,the content of soluble protein and soluble sugar in the seeds of C.guizhouensis had closely relation to the higher calcium content and higher calcium variation content,and the accumulation of characters of macromolecules had position significance for the C.guizhouensis to adapt the higher calcium environment.