Assessment of oxidative damage induced by acute doses of morphine sulfate in postnatal and adult rat brain |
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| Authors: | Guzmán David Calderón Vázquez Ivonne Espítia Brizuela Norma Osnaya Alvarez Raquel García Mejía Gerardo Barragán García Ernestina Hernández Santamaría Daniel de Apreza Mario la Rosa Olguín Hugo Juárez |
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| Institution: | (1) Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), CP 04530 Mexico D.F., Mexico;(2) Laboratorio de Patología Experimental, Instituto Nacional de Pediatría (INP), CP 04530 Mexico D.F., Mexico;(3) Laboratorio de Farmacología, Instituto Nacional de Pediatría , Avenida Imán #1 3er piso, Colonia Cuicuilco, CP 04530 Mexico D.F., Mexico;(4) Departamento de Farmacología, Facultad de Medicina, UNAM, Mexico, Mexico |
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| Abstract: | The aim of the present study is to evaluate the oxidative damage in rats of different ages. Weaned rats of 25 g and adults of 300 g were used in groups of 6, a single i.p. dose of morphine sulfate of 3, 6 or 12 mg/kg was administered. All animals were sacrificed to measure GSH and 5-HT levels in brain by liquid chromatography, as well as Na+, K+-ATPase and total ATPase enzymatic activity. 5-HT levels decreased significantly (p<0.05) in adult animals that received 3 and 6 mg morphine. Na+, K+-ATPase activity increased significantly (p<0.05) in all groups of weaned animals. In adult animals, Na+, K+-ATPase and total ATPase partially diminished. GSH levels diminished significantly (p<0.05) both in weaned and in adult groups. The results indicate age-induced changes in cellular regulation and biochemical responses to oxidative stress induced by morphine. |
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| Keywords: | Brain damage Free radicals Glutathione Morphine Oxidative damage Opioids |
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