Protein phosphorylation in Streptomyces albus |
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Authors: | Z Dobrová M Jirsová T Petík P Ryavý J Náprstek J Janeek |
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Institution: | Institute of Microbiology, Czechoslovak Academy of Sciences, Prague. |
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Abstract: | The phosphorylated proteins of Streptomyces albus, radioactively labeled with 32P]orthophosphate have been analyzed by gel electrophoresis and autoradiography. More than 10 protein species were found to be phosphorylated. With 32P]ATP as substrate cell free extracts phosphorylated endogenous proteins in vitro which were predominantly phosphorylated in vivo. From cell extract which exhibited active phosphorylated in vitro, a protein kinase has been partially purified. The kinase activity was identified in fractions corresponding to a 90 kDa protein. |
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Keywords: | Protein kinase Phosphorylation Streptomycetes |
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