应用DNA分子标记鉴定黄颡鱼、瓦氏黄颡鱼及其杂交种的研究

为区分黄颡鱼（Pelteobagrus fulvidraco Richardson）、瓦氏黄颡鱼（Pelteobagrus vachelli Richardson）及其杂交种，前期研究构建了一个YY超雄黄颡鱼的BAC文库并筛选到了一个包含性别连锁标记Pf62-Y的BAC克隆。研究对该BAC克隆进行测序并鉴定到了一个新基因Inad-like，而且Inad-like的外显子序列在X和Y染色体上基本一致。通过黄颡鱼Inad-like的外显子序列及序列的保守性我们设计引物在瓦氏黄颡鱼中扩增获得了其部分内含子序列。通过内含子序列比对，发现瓦氏黄颡鱼比黄颡鱼少了一个DNA片段。基于黄颡鱼和瓦氏黄颡鱼的显著遗传差异，设计了一对引物，该引物在黄颡鱼和瓦氏黄颡鱼基因组中分别扩增出1908和1178 bp DNA片段，而且在杂交黄颡鱼中同时扩增出这两个DNA片段。总之，这个新的遗传标记提供了一种稳定、高效识别黄颡鱼及其与瓦氏黄颡鱼杂交种的方法。

IDENTIFICATION OF PELTEOBAGRUS FULVIDRACO,PELTEOBAGGRUS VACHELLI AND THEIR INTERSPECIFIC HYBRID BY DNA MARKERS

Yellow catfish (Pelteobagrus fulvidraco) is an important aquacultural fish species in China. Since hydrid breeding is an efficient method to improve aquaculture products, interspecific hybrid between Pelteobagrus fulvidraco and its close species Pelteobaggrus vachelli that grows faster than Pelteobagrus fulvidraco, has been massively pro-duced. However, it is hard to distinguish Pelteobagrus fulvidraco from the interspecific hybrid by morphological fea-tures. Moreover, the interspecific hybrid could not being used as parents for the existence of sterile hybrids. Previously, we constructed a bacterial artificial chromosome (BAC) library from YY super-male yellow catfish and screened out a BAC clone containing the sex-linked marker, Pf62-Y. In this study, we identified a novel gene inad-like in Pelteoba-grus fulvidraco after sequencing the BAC clone, and the exons of inad-like are the same in X and Y chromosomes. Moreover, we cloned partial intron sequences of inad-like in Pelteobaggrus vachelli based on the exon sequences of inad-like in Pelteobagrus fulvidraco. Interestingly, there is a small segment deletion in Pelteobaggrus vachelli in compa-rison with the corresponding sequence in Pelteobagrus fulvidraco. Based on the significant genetic difference between Pelteobagrus fulvidraco and Pelteobaggrus vachelli sequences, we designed one pair of primers, by which 1908 bp and 1178 bp were amplified in Pelteobagrus fulvidraco and Pelteobaggrus vachelli, respectively. Moreover, both 1908 bp and 1178 bp were amplified in the interspecific hybrid. Together, this new genetic marker develops a highly stable and efficient method for distinguishing Pelteobagrus fulvidraco and its interspecific hybrid.