| 定量荧光原位杂交测定端粒长度 |
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| 引用本文： | 曾思聪,卢光琇.定量荧光原位杂交测定端粒长度[J].激光生物学报,2011,20(3):394-397. |
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| 作者姓名： | 曾思聪 卢光琇 |
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| 作者单位： | 中南大学生殖与干细胞工程研究所,人类干细胞国家工程研究中心,湖南长沙410078 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，中国高技术发展研究计划(863计划)，湖南省科技计划项目 |
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| 摘 要： | 目的:应用定量荧光原位杂交(Q-FISH)方法测定端粒长度。方法:选取4种端粒长度均一的标准细胞株采用Q-FISH的方法做出荧光亮度与端粒长度的标准曲线,从而得出实验细胞株的端粒长度,与DNA印迹法测定末端限制性片段(TRF)长度进行二者之间的相关性分析。结果:检测荧光强度的最佳线性曝光时间为400ms,相对于DNA印迹法,定量荧光原位杂交(Q-FISH)法所需标本量少,实验周期短,端粒长度结果与Southern杂交法具有很好的相关性。结论:采用定量荧光原位杂交方法测端粒长度具有重复性好、精确可靠的特点,适用于对珍贵标本的端粒改变进行分析。
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| 关 键 词： | 定量荧光原位杂交 端粒 |
Telomere Length Measurement by Quantitative FISH |
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| ZENG Si-cong,LU Guang-xiu.Telomere Length Measurement by Quantitative FISH[J].ACTA Laser Biology Sinica,2011,20(3):394-397. |
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| Authors: | ZENG Si-cong LU Guang-xiu |
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| Institution: | ZENG Si-cong,LU Guang-xiu~* (Institute of Human Reproduction & Stem Cell Engineering,National Center of Human Stem Cell Research and Engineering,Central South University,Changsha 410078,Hunan,China) |
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| Abstract: | Objective:Our purpose of this study was to set up quantitative florescence suit hybridization(Q-FISH ) to measure telomere length.Methods:4 cell lines which have homogeneous telomere length were selected to establish telomere length standard curve.Telomere length of GM847 cells was evaluated according to the standard curve by Q-FISH or by telomere restriction fragment assay(TRF).Relevance analysis was performed between Q-FISH and TRF experiment group.Results:Our experiments suggest that the best exposure ti... |
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| Keywords: | quantitative florescence suit hybridization telomere |
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