4种鲟鱼养殖亲鱼群体遗传多样性分析

近年鲟鱼人工繁殖技术促进了鲟鱼养殖业的发展,但在选育初期未对后备亲鱼进行遗传背景分析,为了抑制种质资源退化和防止近交衰退,繁育场需要对现有后备亲鱼进行遗传背景分析.本文利用线粒体控制区(D-loop)部分序列对4种养殖鲟鱼后备亲鱼群体(共120个样本)的遗传多样性进行了分析.研究发现不同种鲟鱼D-loop部分序列长度不...

Genetic Diversity of the Reserved Broodstocks in Four Species of Sturgeon

Artificial propagation of Sturgeon has promoted the development of sturgeon aqualture in recent years.However,the genetic relationship among reserved broodstocks has not been analyzed.In order to avoid the degradation of germplasm resources and prevent inbreeding despression,the genetic background of reserved broodstocks should be investigated.In this research,partial sequence of mitochondrial control region(D-loop) was used to examine the genetic diversity of reserved broodstocks in four species of sturgeon.Different length of D-loop sequences between 451-469 bp were obtained from a total of 120 samples.There were 1-18 bp interspecific indels,while no intraspecific indels were detected.Each group contained at least four haplotypes which had greater than 98% sequence similarity.There were 4-12 pasimony informative sites between different haplotypes and genetic distance among intraspecific haplotypes varied from 0.002-0.024.The highest nucleotide diversity was presented in Sterlet(Acipenser ruthenus)(π=0.010) while the lowest was in Amur Sturgeon(A.schrenckii)(π=0.002).The highest haplotype diversity was showed in Siberian Sturgeon(A.baerii)(H=0.706) while the lowest was in Russian Sturgeon(A.gueldenstaedtii)(H=0.352).These results indicate that there is a low genetic diversity in the four reserved broodstocks and that inbreeding impact and genetic distance should be seriously considered in the fry production and germplasm conservation of the four species of sturgeon.