Root herbivory in vitro: Interactions between roots and aphids grown in aseptic coculture |
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Authors: | Tianshu Wu Jonah Wittkamper Hector E Flores |
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Institution: | (1) Department of Plant Pathology, The Pennsylvania State University, 313 Wartik Laboratory, 16802 University Park, Pennsylvania;(2) Williams College, SU Box 3098, 01267 Williamstown, Massachusetts |
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Abstract: | Summary An in vitro coculture system has been established to study interactions between roots and aphids. Eight aphid species (Aphis spiraecola P., Trama rara M., Macrosiphum euphorbiae S., Rhopalosiphum padi L., Sitobion avenae F., Rhopalosiphum maidis F., Metopolophium dirhodum W., and Pemphigus populivenae F.) were reared on six species of hairy root cultures, Carthamus tinctorius L. cv N10, Tagetes patula L., Trichosanthes cucumerina L. var anguina, Hyoscyamus muticus L., Nicotiana tabacum L., and Beta vulgaris L. subsp. vulgaris. All species of aphids survived on root cultures for at least 2 d. Three cocultures have been maintained aseptically for
periods ranging from 2 mo. to over 2 yr. The coculture of R. padi on C. tinctorius cv N10 (N10-Rp) was used to study morphological and biochemical responses of roots under aphid herbivory. Aphid herbivory
caused browning of cultures, reduced root vegetative growth, and increased production of polyacetylenes in C. tinctorius cv N10 roots. Our results suggest that this coculture system may improve our understanding of interactions between aphids
and plant roots. |
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Keywords: | hairy root root culture aphid coculture herbivory polyacetylene interaction |
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