| 一氧化氮在光延缓小麦叶片衰老中的作用 |
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| 引用本文： | 张爱玲,贺军民.一氧化氮在光延缓小麦叶片衰老中的作用[J].西北植物学报,2009,29(3). |
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| 作者姓名： | 张爱玲 贺军民 |
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| 作者单位： | 陕西师范大学,生命科学学院,西安,710062 |
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| 摘 要： | 以小麦(Triticum aestivum L.)离体叶片为材料,采用药理学实验和激光扫描共聚焦显微镜技术,对内源一氧化氮(NO)在光延缓离体小麦叶片衰老过程中的作用进行了研究.结果显示:光照处理的同时添加NO清除剂血红蛋白(Hb)或硝酸还原酶抑制剂钨酸钠(Na2WO4)后,光抑制离体小麦叶片叶绿素和可溶性蛋白含量降低及丙二醛累积的效应均显著减弱;光照处理下小麦叶片硝酸还原酶活性和内源NO水平均明显高于黑暗条件下,而Na2WO4处理不仅能抑制光诱导的硝酸还原酶活性提高,还与Hb处理一样能显著降低光下叶片内源NO水平.结果表明,硝酸还原酶途径来源的NO参与了光抑制离体小麦叶片衰老的过程.
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| 关 键 词： | 一氧化氮 硝酸还原酶 光 衰老 小麦叶片 |
The Role of Nitric Oxide in Light-inhibited Senescence of Wheat Leaves |
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| ZHANG Ai-ling,HE Jun-min.The Role of Nitric Oxide in Light-inhibited Senescence of Wheat Leaves[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(3). |
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| Authors: | ZHANG Ai-ling HE Jun-min |
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| Institution: | College of Life Sciences;Shaanxi Normal University;Xi'an 710062;China |
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| Abstract: | By pharmacological experiments combined with laser-scanning confocal microscopy,the role and enzymic source of nitric oxide(NO) in light-inhibited senescence of the detached wheat(Triticum aestivum L.) leaves were studied.Compared to light treatment alone,the treatment of light combined with the NO scavenger hemoglobin(Hb) or nitrate reductase(NR) inhibitor sodium tungstate(Na2WO4) reduced the contents of chlorophyll and soluble protein,and increased lipid peroxidation as quantified by measurement of malond... |
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| Keywords: | nitric oxide nitrate reductase light senescence wheat leaves |
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