| 激光对香石竹愈伤组织蛋白质和同工酶谱的影响 |
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| 引用本文： | 陈汝民,刘颂豪.激光对香石竹愈伤组织蛋白质和同工酶谱的影响[J].激光生物学报,1992,1(2):62-66. |
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| 作者姓名： | 陈汝民 刘颂豪 |
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| 作者单位： | 华南师范大学生物系激光生命科学研究中心 广州
(陈汝民)，华南师范大学生物系激光生命科学研究中心 广州(刘颂豪) |
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| 摘 要： | 用氦氖激光处理香石竹愈伤组织,可使材料中的34kD和28kD蛋白含量高于对照,并产生分子量约为22kD的新的蛋白带。而用氩离子激光处理后,则出现一条分子量约为45kD的新的蛋白带。此外,激光处理亦明显影响愈伤组织的过氧化物酶同工酶谱及其活性,其中氦氖激光处理后产生了Rf为0.17和0.26的新酶带,而用氩离子激光处理则只产生Rf为0.17的新酶带。激光对香石竹愈伤组织的酯酶同工酶无影响。
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| 关 键 词： | 激光 香石竹 愈伤组织 蛋白 |
EFFECTS OF LASER RADIATION ON THE PROTEIN AND ISOENZYME OF THE CALLUS OF CLOVE GILLY-FLOWER |
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| Chen Rumin Liu Songhao.EFFECTS OF LASER RADIATION ON THE PROTEIN AND ISOENZYME OF THE CALLUS OF CLOVE GILLY-FLOWER[J].ACTA Laser Biology Sinica,1992,1(2):62-66. |
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| Authors: | Chen Rumin Liu Songhao |
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| Institution: | Chen Rumin Liu Songhao
(Biology Department Laser and Life Science Reasearch Center) (South China Normal University) |
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| Abstract: | When the callus of Clove Gilly was treated with He-Ne laser (20 mW), the protein that MW were 34 kD and 28 kD, respectively,increased and a kind of new protein(MW 22 KDa) appeared.A kind of proteia(MW 45 kD) was produced when the callus was treated with Ar + -laser ( 0.1W and lW). Furthermore, the bands and activity of peroxidase isoenzyme which the callus was treated with He-Ne laser were apparently affected, two brands which Rf volues were 0.17 and 0.26, respectively, appeared, but only a brand ( Rf-0.17) appeared, under the treatment of Ar + -laser. There was not effect on esterase isoenzyme in the callus which treated by laser. |
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| Keywords: | laser clove gilly-flower callus prptein isoenzyme |
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