几种基因芯片技术的比较

基因芯片技术因其自身的优越性将成为基因组序列分析和基因表达研究的主要工具。但是由于不同的实验室使用不同的技术平台 ,从 2 5到 80个碱基的寡核苷酸芯片和长度从数百到几千个碱基的cDNA阵列。不同技术平台的存在 ,为芯片资料的利用和芯片领域的标准化分析带来了困难。通过对不同技术平台的研究比较 ,短的和长的寡核苷酸芯片技术具有明显的优势 ,可望成为将来主要的技术方法。

Comparison of Genechip Platforms

Gene chip promises to be a revolutionary tool for large scale parallel anaylsis of genome sequence and gene expression because of its advantages. However, owing to the different microarray platforms, which can contain oligonucleotides of 25 to 80 bases in length and cDNAs of hundreds of bases to several kilobases in length, there are many difficulties in application of microarray data widely and standardization of microarray field. Comparion of several genechip platforms, short and long oligonucleotide technologies offer advantages, and could possibly become the major competing platforms in the near future.;