大花蕙兰组培快繁技术研究

选用大花蕙兰杂交新品种1053为外植体,采用1/2MS为基本培养基进行大花蕙兰组培快繁技术研究,结果表明：类原球茎诱导的最佳外植体为大花蕙兰鳞茎,培养基最佳激素浓度配比为0.5 mg/L 6-BA＋0.2 mg/L NAA;类原球茎增殖与分化芽最佳激素浓度配比为1.0 mg/L 6-BA＋0.3 mg/L NAA;幼芽增殖的最佳激素浓度配比为2.0 mg/L 6-BA＋0.5 mg/L NAA,最佳有机添加物为150 ml/L椰汁,幼芽增殖正交试验得出影响因素主次顺序：6-BA〉椰汁添加物〉NAA,优组合为2 mg/L 6-BA、0.5 mg/L NAA、添加物椰汁150 ml/L;诱导生根的最佳激素浓度配比为0.3 mg/L IBA＋0.2 mg/L NAA,添加香蕉泥100 g/L对幼苗根生长有显著的促进作用。优化后的大花蕙兰组培快繁技术参数,可为大规模工厂化生产提供参考。

Research on rapid propagation technology of tissue culture of Cymbidium

The 1053 freshmen Cymbidium seedling variety were chosen as explants, using MS as basic medium for tissue culture of Cymbidium. Experiment results show that PLB - induced is optimal explant for the Cymbidium bulbs, the optimal ratio of hormone con- centrations is 0.5mg/L 6-BA ＋ 0.2mg/L NAA. PLB optimal hormone concentration ratio of proliferation of protocorm and differentia- tion of bud is 1. Omg/L 6-BA ＋ 0.3mg/L NAA; the bud growth hormone concentration ratio is 2.0mg/L6-BA ＋ 0.5mg/LNAA; organic additive is 150ml / L coconut. The bud proliferation orthogonal test results show that primary and secondary order are 6-BA 〉 coconut additives 〉 NAA, suitable combination is 2mg/L 6-BA, 0.5mg/L NAA, coconut milk additives 150ml / L. The rooting hor- mone concentration ratio is 0.3mg/L IBA ＋ O. 2mg/L NAA, adding lO0g/L banana mud has significant effect on the growing of the seedling root.