| 基于ISSR标记的辽宁4个东北林蛙种群遗传多样性分析 |
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| 引用本文： | 周瑜,杨宝田,陆宇燕,巴山,周正彦,李丕鹏.基于ISSR标记的辽宁4个东北林蛙种群遗传多样性分析[J].四川动物,2011,30(3). |
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| 作者姓名： | 周瑜 杨宝田 陆宇燕 巴山 周正彦 李丕鹏 |
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| 作者单位： | 辽宁省生物进化与多样性重点实验室,沈阳师范大学化学与生命科学学院,沈阳,110034 |
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| 基金项目： | 国家自然科学基金，辽宁省自然科学基金 |
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| 摘 要： | 辽宁是东北林蛙Rana dybowskii主要分布地之一,种群数量巨大,其群体遗传多样性有待评估.本研究应用ISSR标记技术对东北林蛙4个种群105个样本进行研究,5个引物共获得44条清晰谱带,4个种群的多态位点率均大于75%,Nei's基因多样性为0.2851,Shannon信息指数为0.4476,显示了较高的遗传多样性.对遗传分化系数、Nei's遗传距离、AMOVA分子变异巢式方差分析和F-统计量等遗传参数的统计结果表明,辽宁东北林蛙种群间已经出现一定程度的遗传分化,分析认为,自然屏障(高山和平原等)以及栖息地片段化是其遗传分化形成的主要因素.
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| 关 键 词： | 东北林蛙 遗传多样性 遗传分化 |
Genetic Diversity of Rana dybowskii among Four Local Populations in Liaoning Province Based on ISSR Markers |
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| ZHOU Yu,YANG Bao-tian,LU Yu-yan,BA Shan,ZHOU Zheng-yan,LI Pi-peng.Genetic Diversity of Rana dybowskii among Four Local Populations in Liaoning Province Based on ISSR Markers[J].Sichuan Journal of Zoology,2011,30(3). |
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| Authors: | ZHOU Yu YANG Bao-tian LU Yu-yan BA Shan ZHOU Zheng-yan LI Pi-peng |
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| Institution: | ZHOU Yu,YANG Bao-tian,LU Yu-yan,BA Shan,ZHOU Zheng-yan,LI Pi-peng (Liaoning Key Laboratory of Evolution and Biodiversity,Shenyang Normal University,Shenyang 110034,China) |
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| Abstract: | Genetic diversity among 4 local populations of Rana dybowskii distributed in Liaoning province was analyzed using inter-simple sequence repeat (ISSR) markers.Five primers were used to amplify 105 individuals,and 44 bands were clearly tested.The populations of R.dybowskii showed a comparatively high genetic diversity.Among 4 local populations,the percentage of polymorphic loci was above 75%,Nei's gene diversity and Shannon index was 0.2851 and 0.4476,respectively.The result of Gst,Nei's genetic distance,anal... |
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| Keywords: | ISSR |
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