| 显微CT在骨微结构研究中的应用 |
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| 引用本文： | 王维刚,刘震泽,李俊,严惠敏.显微CT在骨微结构研究中的应用[J].中国细胞生物学学报,2012(1):16-22. |
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| 作者姓名： | 王维刚 刘震泽 李俊 严惠敏 |
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| 作者单位： | 同济大学化学系;同济大学生命科学与技术学院;上海南方模式生物研究中心 |
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| 基金项目： | 国家高技术研究发展计划(No.2008AA02Z126);上海市科委项目(No.10140901400)资助项目~~ |
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| 摘 要： | 该文对OPG基因敲除小鼠进行micro-CT扫描,进行全身骨骼三维重建,并分析股骨结构。结果发现OPG基因敲除纯合子小鼠全身骨骼出现弥漫性蜂窝状低密度影。OPG基因敲除小鼠的骨密度、骨结构模型指数、骨小梁厚度、骨体积分数、骨表面积与体积比、连接密度以及皮质骨厚度等指标均明显小于同窝野生型小鼠,而两基因型小鼠在骨小梁数目、骨小梁分离度及各项异性度等方面无明显差异。以上数据结果表明,OPG基因敲除导致小鼠出现骨质疏松。应用micro-CT可以对小鼠骨进行无创微结构分析,较体视学方法具有一定的优势。
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| 关 键 词： | 显微CT 骨微结构 骨质疏松 骨保护素 基因敲除小鼠 |
The Use of Micro-CT in the Study of Bone Microarchitecture |
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| Authors: | Wang Weigang Liu Zhenze Li Jun Yan Huimin |
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| Institution: | 1Department of Chemistry,Tongji University,Shanghai 200092,China;2School of Life Science and Technology,Tongji University,Shanghai 200092,China;3Shanghai Research Center for Model Organisms,Shanghai 201203,China) |
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| Abstract: | OPG knockout mice were scanned by micro-CT.The reconstruction of the three-dimension mouse skeleton showed the widespread honeycomb shaped low density areas in OPG knockout mice.And we found that OPG knockout mice with lower level of BMD,SMI,Calibration of trabecular thickness,BV/TV,BS/BV,connectivity density and cortical bone thickness in comparison to wild-type littermates.But there was no any significant differences in the trabecular number,trabecular separation or DOA in both genotypes.The deletion of OPG in mice leads to osteoporosis.Micro-CT can be used to study bone architecture noninvasively in mice with some advantage over stereological measures. |
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| Keywords: | micro-CT bone microarchitecture osteoporosis osteoprotegerin knockout mouse |
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