Cellulose and xylans in the interface capsule in symbiotic cells of actinorhizae |
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Authors: | R H Berg |
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Institution: | (1) Biology Department, Memphis State University, 201 Life Sciences Bldg., 38152 Memphis, TN, USA |
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Abstract: | Summary Enzyme-gold affinity labeling was used to show that in mature infected cells of actinorhizal symbioses the capsule on the plant host side of the symbiotic interface contained cellulose and xylans. Host species examined for cellulose wereAlnus rubra, Casuarina equisetifolia, C. glauca, Ceanothus cuneata, C. velutinus, Elaeagnus pungens, andMyrica cerifera.. Cellulose was in the capsule throughout the infected cell, implying that during development cellulose synthase was present in the host cell membrane component of the symbiotic interface. Any possible degradation of capsule cellulose by the microsymbiont was either incomplete or transient, because the polymer was present in mature infected cells. Cellulose labeling inCeanothus andElaeagnus was less consistent than in the other species. Dual labeled capsules inCasuarina glauca andAlnus rubra showed a similar distribution of xylans and cellulose. Cytochemical studies indicate that the capsule contains three major classes of cell wall polysaccharides: cellulose, hemicellulose (xylans), and pectins (shown previously). This suggests that the capsule is essentially a thin, internal, tubular plant cell wall.Abbreviations Au5
Au15 colloidal gold particles with mean diameter of 5 and 15 nm, respectively
- CBHI
cellobiohydrolase I
- CBHII
cellobiohydrolase II
- PBS
phosphate-buffered saline |
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Keywords: | Actinorhizal capsule Cellulose Enzyme-gold cytochemistry Frankia Plant cell wall Xylans |
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