耐极端环境真菌对犬小孢子菌及孢子丝菌的拮抗研究

目的 检测极端环境分离出的真菌拮抗临床病原真菌的活性.方法 选用极端环境分离出的24株真菌菌株上清液及菌丝体的提取液,来进行临床病原真菌（孢子丝菌和犬小孢子菌）的拮抗试验,采用M38-A2产孢丝状真菌抗真菌药物敏感性试验方案和E-test纸片扩散法.结果 抑菌圈直径≥1.0 cm,具有较强拮抗孢子丝菌活性的极端环境真菌有8株;抑菌圈直径≥2.0 cm,具有极强拮抗犬小孢子菌的活性的极端环境真菌有1株.两种临床菌株的拮抗试验,90％的拮抗结果相一致.结论 70％极端环境真菌菌株上清液及菌丝体提取液具有拮抗临床病原真菌孢子丝菌和犬小孢子菌的活性.

Antagonistic activity of extreme environment fungal isolates to Microsporum canis and Sporothrix sp.

Objective To detect antagonistic activity of fungal strains isolated from the extreme environment against Microsporum canis and Sporothrix.Method The 24 fungal strains were isolated from the extreme environment,and then the supernatants of their cultures were extracted,for exploring the antagonism for clinical pathogenic fungi such as Microsporum canis and Sporothrix sp.according to M38-A2 and E-test criterion.Results A total of 8 fungal strains＇supernatants showed that diameters of bacteriostatic circles are equal or greater than 1.0 cm,which revealed evident antagonistic activity to Sporothrix sp..Similarly,9 fungal strains＇supernatants show evident antagonistic activity to Microsporum canis due to the diameters of bacteriostatic circles are equal or greater than 1.0 cm.The antagonism results revealed 90% of correlation in antagonism test using these two different fungal species.Seventy pencents of the fungal supernatants and the mycelium extracts of extreme environment fungi showed antagonistic activity for Microsporum canis and Sporothrix sp..Conclusion Some extreme environment fungi have antagonistic activites for Microsporum canis and Sporothrix.