QTL Analysis in Recombinant Chromosome Substitution Lines and Doubled Haploid Lines Derived from a Cross between Hordeum vulgare ssp. vulgare and Hordeum vulgare ssp. spontaneum |
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Authors: | Kiyosumi Hori Kazuhiro Sato Nami Nankaku and Kazuyoshi Takeda |
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Institution: | (1) Research Institute for Bioresources, Okayama University, 710-0046 Chuo, Kurashiki, Japan |
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Abstract: | Recombinant chromosome substitution lines (RCSLs) were developed in BC3 generation to introduce segments of a wild barley strain ‘H602’ (Hordeum vulgare ssp. spontaneum) into a barley cultivar ‘Haruna Nijo’ (H. vulgare ssp. vulgare) genetic background. One hundred thirty four RCSLs were genotyped by 25 SSR and 60 EST markers, which were localized on a
linkage map of doubled haploid lines (DHLs) derived from the same cross combination. Graphical genotyping revealed that the
observed average substitution ratio of H602 segment (12.9%) agreed with the expected substitution ratio (12.5%), and a minimum
set of 19 RCSLs represented the entire H602 genome. Phenotypes of five qualitative and nine quantitative traits were scored
in both the RCSLs and DHLs. Five qualitative traits were localized as morphological markers on the linkage map of the DHLs,
and these molecular markers were aligned on the respective chromosomal regions in the RCSLs. Simple and composite interval
mapping procedures detected a total of 18 and 24 QTLs for nine qualitative traits on the RCSLs and DHLs, respectively. Several
QTLs were localized at coincident or very close regions on both linkage maps. In spite of general inferior agronomic performances
in wild barley, several H602 QTL alleles showed agronomically positive effects. These RCSLs should contribute to substitution
of favorable alleles from wild barley into cultivated barley. These RCSLs are also available as sources of near isogenic lines,
with which we can apply advanced genetic analysis methods such as isolation of QTLs and detection of epistatic interactions
among QTLs. |
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Keywords: | AB-QTL analysis Barley Hordeum vulgare L QTL Recombinant chromosome substitution lines (RCSLs) |
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