白藜芦醇对AngⅡ诱导的血管平滑肌细胞CaN的影响

目的：观察白藜芦醇（Res）对血管紧张素Ⅱ（AngⅡ）诱导的血管平滑肌细胞（VSMCs）增殖及细胞中钙调蛋白（CaM）和钙调神经磷酸酶（CaN）活性的影响,并探讨其机制。方法：体外培养兔主动脉VSMCs,用免疫细胞化学方法鉴定。建立AngⅡ诱导的VSMCs增殖模型。取生长良好的第4～8代VSMCs,随机分为对照组,AnsⅡ组（0．1μmol／L）,AngⅡ＋Res组（20,40,80,160）μmol／L。应用MTT法检测细胞增殖程度,考马斯亮兰法进行CaM定量,定磷法进行CaN活性测定。结果：成功培养兔VSMCs并传代,免疫细胞化学染色均呈阳性表达。AngⅡ组VSMCs的增殖程度、CaM和CaN活性较对照组增高（P〈0．05,P〈0．01）。AngⅡ＋Res组各组VSMCs的CaM和CaN活性较AngⅡ组显著下降（P〈0．01）。结论：在一定范围内,Res可降低AngⅡ诱导的VSMCs增殖程度,其机制可能与干预CaN依赖的信号转导途径有关。

Effects of resveratrol on the proliferation and can of vascular smooth muscle cell induced by angiotensin II

Aim： To investigate the effects of resveratrol（Res） on the proliferation of VSMCs induced by Ang Ⅱ and the expression of calmodulin （CaM） and calcineurin（CaN） in the proliferation of VSMCs treated by AngⅡ and to discuss the mechanism. Methods： Rabbit arterial VSMCs were cultured in vitro and VSMCs were identified with the method of immunocytochemistry. A cell proliferating model of VSMCs induced by AngⅡ was established. VSMCs were cultured for 4 - 8 passages. The experiments were randomly divided into control group, AngⅡ group （0.1 μmoL/L） and AngⅡ ＋ Res groups with different concentrations（20, 40, 80, 160）μmol/L. VSMCs proliferration was determined with MTT colorimetric method. CaM was detected with Coomassie brilliant blue method and CaN was determined by enzyme reaction phosphorus measurement. Results： Rabbit VSMCs were cultured successfully and could be passaged. After immunocytochemistry staining, all the cells cytoplasm were stained and positive. Cell proliferation, CaM and CaN activities were increased significantly in VSMCs proliferation induced by AngⅡ（ P 〈 0.05, P 〈 0.01）. The index of Ang Ⅱ ＋ Res groups were obviously reduced compared with AngⅡ group（ P 〈 0.01）. Conclusion： The VSMCs proliferation induced by Ang Ⅱ can be inhibited by Res significantly, and the inhibiting mechanism of Res may be related to inhibiting CaM and CaN activities then restraining the proliferation of VSMCs in a dose dependent manner.