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橡胶树种质资源遗传多样性研究——Ⅰ.速生种质遗传多样性RAPD分析
引用本文:安泽伟,黄华孙,姚庆收,方家林.橡胶树种质资源遗传多样性研究——Ⅰ.速生种质遗传多样性RAPD分析[J].植物遗传资源学报,2004,5(2):128-132.
作者姓名:安泽伟  黄华孙  姚庆收  方家林
作者单位:中国热带农业科学院橡胶研究所/农业部热带作物栽培生理学重点开放实验室/国家橡胶树育种中心 海南儋州571737 (安泽伟,黄华孙,姚庆收),中国热带农业科学院橡胶研究所/农业部热带作物栽培生理学重点开放实验室/国家橡胶树育种中心 海南儋州571737(方家林)
基金项目:中国热带农业科学院科技基金项目资助
摘    要:应用RAPD技术对8份野生种质和12份栽培种质进行遗传多样性分析,筛选到18个具有多态性扩增的引物.共扩增出128条带。据Nei-Li相似系数将20份材料分别聚为野生种质和栽培种质两大类。5个野生种质聚为野生种质类群,12个栽培种质和3个野生种质聚为栽培种质类群。研究结果表明,RAPD技术用于橡胶树种质资源研究,能够为野生种质优良特性导入栽培种质提供分子水平的参考依据。

关 键 词:橡胶树  种质资源  遗传多样性  分子标记  RAPD
修稿时间:2003年10月27

Study on Genetic Diversity of Hevea brasiliensis (Rubber tree) I. Analysis for Genetic Diversity of Rubber Tree with High Annual Growth Using RAPD
AN Ze wei,HUANG Hua sun,YAO Qing shou,FANG Jia lin.Study on Genetic Diversity of Hevea brasiliensis (Rubber tree) I. Analysis for Genetic Diversity of Rubber Tree with High Annual Growth Using RAPD[J].Journal of Plant Genetic Resources,2004,5(2):128-132.
Authors:AN Ze wei  HUANG Hua sun  YAO Qing shou  FANG Jia lin
Abstract:The genetic diversity of rubber tree was analyzed by using RAPD. There were 18 primers showing abundant polymorphisms. The 18 primers produced 128 polymorphic bands. The results of cluster analysis based on Nei Li coefficient revealed that these 20 samples were divided into two main groups. Five wild germplasm were clustered into wild germplasm group, and 12 cultivated germplasm and three wild germpladm were clustered into cultivated germplasm group. The results showed that RAPD marker was suitable for study on genetic diversity in rubber tree, and offering the molecular basis, on which the genetic substance of wild rubber tree was transferred to cultivated rubber tree.
Keywords:Rubber tree  Germplasm resources  Genetic diversity  Molecular marker  RAPD
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