Population genetic structure of the medicinal plant Vitex rotundifolia in China: implications for its use and conservation |
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Authors: | Hu Yuan Zhu Yu Zhang Qiao-Yan Xin Hai-Liang Qin Lu-Ping Lu Bao-Rong Rahman Khalid Zheng Han-Chen |
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Institution: | Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. |
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Abstract: | Vitexrotundifolia L.is an important plant species used in traditional Chinese medicine.For its efficient use and conservation,genetic diversity and clonal variation of V.rotundifolia populations in China were investigated using inter-simple sequence repeat markers.Fourteen natural populations were included to estimate genetic diversity,and a large population with 135 individuals was used to analyze clonal variation and fine-scale spatial genetic structure.The overall genetic diversity (GD) of V.rotundifolia populations in China was moderate (GD=0.190),with about 40% within-population variation.Across all populations surveyed,the average within-population diversity was moderate (P = 22.6%; GD = 0.086).A relatively high genetic differentiation (Gst=0.587)among populations was detected based on the analysis of molecular variance data.Such characteristics of V.rotundifolia are likely attributed to its sexual/asexual reproduction and limited gene flow.The genotypic diversity (D=0.992) was greater than the average values of a clonal plant,indicating its significant reproduction through seedlings.Spatial autocorrelation analysis showed a clear within-population structure with gene clusters of approximately 20 m.Genetic diversity patterns of V.rotundifolia in China provide a useful guide for its efficient use and conservation by selecting particular populations displaying greater variation that may contain required medicinal compounds,and by sampling individuals in a population at >20 m spatial intervals to avoid collecting individuals with identical or similar genotypes. |
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Keywords: | beach vitex medicinal plant conservation Vitex rotundifolia gene cluster genetic diversity sampling strategy spatial structure |
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