| 从盾叶薯蓣组培苗中高压酸解制备薯蓣皂苷元 |
| |
| 引用本文： | 阴春晖,李培琴,赵江林,单体江,何桂玲,周立刚.从盾叶薯蓣组培苗中高压酸解制备薯蓣皂苷元[J].天然产物研究与开发,2011,23(1):114-117. |
| |
| 作者姓名： | 阴春晖 李培琴 赵江林 单体江 何桂玲 周立刚 |
| |
| 作者单位： | 中国农业大学农学与生物技术学院,北京,100193 |
| |
| 基金项目： | 北京市自然科学基金，国家自然科学基金 |
| |
| 摘 要： | 采用正交试验法对盾叶薯蓣(Dioscorea zingiberensis)组培苗中薯蓣皂苷元的高压酸解制备工艺进行了研究。以薯蓣皂苷元的含量作为评价指标,选用正交表L16(45),以样品用量、硫酸浓度、提取时间为因素,设计了3因素4水平的正交试验。结果表明:高压酸解提取薯蓣皂苷元的最佳工艺条件为:样品用量25 mg、硫酸浓度0.5 mol/L、提取时间2 h,在此条件下提取物中薯蓣皂苷元的平均含量为9.12 mg/g。
|
| 关 键 词： | 盾叶薯蓣 薯蓣皂苷元 高压酸解 正交试验 |
Acidolysis Preparation of Diosgenin at High Pressure from the Cultured Plantlets of Dioscorea zingiberensis |
| |
| YIN Chun-hui,LI Pei-qin,ZHAO Jiang-lin,SHAN Ti-jiang,HE Gui-ling,ZHOU Li-gang.Acidolysis Preparation of Diosgenin at High Pressure from the Cultured Plantlets of Dioscorea zingiberensis[J].Natural Product Research and Development,2011,23(1):114-117. |
| |
| Authors: | YIN Chun-hui LI Pei-qin ZHAO Jiang-lin SHAN Ti-jiang HE Gui-ling ZHOU Li-gang |
| |
| Institution: | YIN Chun-hui,LI Pei-qin,ZHAO Jiang-lin,SHAN Ti-jiang,HE Gui-ling,ZHOU Li-gang College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China |
| |
| Abstract: | Acidolysis preparation of diosgenin at high pressure from the cultured plantlets of Dioscorea zingiberensis was studied by L16(45) orthogonal experiments.The effects of sample quantity,concentration of sulfuric acid,and extraction time were investigated.The results showed that diosgenin content of the plantlets reached 9.12 mg/g under the optimum extraction condition that was 25 mg of sample,0.5 mol/L of sulfuric acid,and 2 h of extraction time. |
| |
| Keywords: | Dioscorea zingiberensis diosgenin acidolysis preparation at high pressure orthogonal experiments |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
