| 巴西固氮螺菌Yu62在玉米根的定植 |
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| 引用本文： | 刘元,陈三凤,李季伦.巴西固氮螺菌Yu62在玉米根的定植[J].Acta Botanica Sinica,2003,45(6):748-752. |
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| 作者姓名： | 刘元 陈三凤 李季伦 |
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| 作者单位： | 中国农业大学生物学院，中国农业大学生物学院，中国农业大学生物学院 农业生物技术国家重点实验室，农业微生物资源及其应用实验室，北京100094，农业生物技术国家重点实验室，农业微生物资源及其应用实验室，北京100094，农业生物技术国家重点实验室，农业微生物资源及其应用实验室，北京100094 |
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| 基金项目： | 国家高技术研究发展计划“863”项目(2 0 0 1AA2 14 0 2 1)，国家重点基础研究发展规划项目 (0 0 1CB10 890 4)~~ |
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| 摘 要： | 将GFPmut2质粒中的gfp基因(编码绿色荧光蛋白)克隆到载体pVK100中，构建成重组质粒pVK1001。将pVK1001通过电转化方法导入到联合固氮菌巴西固氮螺菌Yu62中，获得GFP)标记的巴西固氮螺菌Yu62菌株。用标记菌株接种限菌培养条件下生长的玉米(农大3318)幼苗，在接种后8d、12d，用激光共聚焦扫描显微镜进行观测，结果表明巴西固氮螺菌Yu62菌株能定植于玉米根部皮层的薄壁细胞间隙。用扫描电镜和超薄切片电镜观察表明，大多数细菌主要定植于根表，少数菌可进入玉米根组织内。
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| 关 键 词： | 绿色荧光蛋白 巴西固氮螺菌 Yu62 定植 激光共聚焦扫描显微镜 透射电子显微镜 |
Colonization Pattern of Azospirillum brasilense Yu62 on Maize Roots |
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| Authors: | LIU Yuan CHEN San_Feng LI Ji_Lun |
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| Institution: | LIU Yuan,CHEN San_Feng *,LI Ji_Lun |
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| Abstract: | Plasmid pVK1001 which carried the gfp gene of GFPmut2, a mutant of GFP, was introduced into Azospirillum brasilense Yu62 by electroporation. Maize seedlings were inoculated with the GFP_labelled bacteria and grown gnotobiotically in flask with semi_solid agar medium. Observations were performed with confocal laser scanning microscopy (CLSM) and electron microscopy, respectively, at 8 d and 12 d after inoculation. Confocal laser scanning microscopy showed that A. brasilense Yu62 could penetrate into the cortex tissue, colonizing in the intercellular spaces of the parenchyma cells of the cortex tissue. Transmission and scanning electron microscopy (TEM) showed that the majority of the bacteria colonized on the root surface and only a minority of them resided in the root interior. |
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| Keywords: | green fluorescent protein (GFP) Azospirillum brasilense Yu62 colonization confocal laser scanning microscopy (CLSM) transmission electron microscopy (TEM) scanning electron microscopy (SEM) |
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