北柴胡种子内生菌群落结构与多样性

为研究北柴胡种子内生菌的群落结构与多样性.采用Illumina Miseq高通量测序技术,分别对山西(BC_1)、黑龙江(BC_2)、河北(BC_3)和内蒙古(BC_4)的四个产地(4份)北柴胡种子的16S RNA V3-V4区和ITS1区扩增片段进行测序,并对内生细菌和内生真菌群落结构和多样性进行分析.结果表明,BC...

Community structure and diversity of endophytes of Bupleurum chinense DC.seeds

In order to analyze the species abundance and diversity of endophytes in Bupleurum chinense DC. seeds,the amplified fragments of 16 S RNA V3-V4 region and ITS1 region of representative Bupleurum chinense DC. seeds from Shanxi(BC＿1),Heilongjiang(BC＿2),Hebei(BC＿3)and Inner Mongolia(BC＿4)were sequenced by Illumina Miseq high-throughput sequencing technique,and the community structure and diversity of endophytic bacteria and fungi were analyzed. The results showed that the richness and diversity of endophytic bacteria and fungi in BC＿3 was the highest,while the richness of endophytes in BC＿4 was the lowest. The dominant phylum of endophytic bacteria in Bupleurum chinense DC. seeds from four different habitats were Proteobacteria(77. 77%),and the dominant phylum of endophytic fungi were Basidiomycota(67. 82%). The dominant genus of endophytic bacteria was Sphingomonas(18. 30%),and the dominant genus of endophytic fungi was Papiliotrema(41. 35%). The high abundance of endophytic fungi was Papiliotrema aurea(38. 67%)and the high abundance of endophytic bacteria was Pseudomonas rhizosphaerae(15. 56%).There were differences in the community structure of endophytic bacteria and fungi in the B. chinense DC. seeds from four different habitats at the genus level. The endophytes in the B. bupleurum DC. seeds are rich in resources and diverse in functions,which have an important impact on seed germination,growth and development.