宫颈癌中p53表达和病毒感染的研究

应用PCR对121例宫颈脱落细胞和组织分别检测HPV-16／18和HSV-2DNA。同时应用免疫组化S-P法检测76例宫颈组织中P53过度表达。结果发现，宫颈癌组织中HPV-16／18和HSV-2阳性率分别为61．3％和32．3％，慢性宫颈炎组HPV-16／18和HSV-2阳性率分别为22．5％和20．0％，与正常宫颈组比较均有显著性差异。宫颈癌中HPV-16／18和HSV-2混合感染率为16．1％。p53过度表达率在慢性宫颈炎、宫颈上皮内瘤变(CIN)、宫颈癌组织中呈梯度递增。另外，宫颈癌组织中P53过度表达与HPV-16／18、HSV-2的感染无相关性。提示：宫颈癌的发生与HPV-16／18关系密切，HSV-2可能与HPV-16／18协同作用导致宫颈癌的发生。宫颈组织中p53过度表达率与宫颈癌的进程有关，这在宫颈癌的防治方面有一定的临床意义。

p53 Expression and Genetic Evidence for Virus Infection in Cervical Carcinoma

We examined 121 Specimens of exfoliated cells and tissue of cervix for the presence of HPV-16/18 and HSV-2 DNA by PCR. At the same time, we analysised 76 specimens from tissue of cervix for the expression of p53 by immunohistochemical assay. The HPV-16/18 was found in 19 (61.3%) of 31 cervical carcinoma, in 9 (22.5%) of 40 chronic cervicitis, in 3 (6.0%) of 50 normal uterine cervix. The positive rate of HSV-2 was 32.3% (10/31), 20.0% (8/40) and 4.0% (2/50). The mixed infection rate of HPV-16/18 and HSV-2 was 16.1% in cervical carcinoma. The rates of positive expression of p53 was found to have a gradient of crescendo in the tissues of chronic cervicitis, CIN and cervical carcinoma. We found that cervical carcinoma is strongly related with HPV-16/18 infection. A synergetic action may occur between HPV and HSV-2 infections in the oncogenesis of cervical carcinoma. The overepression of p53 appears to be closely associated with the development of cervical cancer. There is a matter of clinical significance on the prevention and treatment of cervical carcinoma.