| 脑缺血后大鼠神经元和星形胶质细胞P21cip1表达的研究 |
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| 引用本文： | 陈华先,朱舟,王萍,张贵斌,喻志源,谢敏杰,徐运兰,王伟.脑缺血后大鼠神经元和星形胶质细胞P21cip1表达的研究[J].中国组织化学与细胞化学杂志,2007,16(6):660-663. |
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| 作者姓名： | 陈华先 朱舟 王萍 张贵斌 喻志源 谢敏杰 徐运兰 王伟 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 |
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| 摘 要： | 目的研究大鼠局灶性脑缺血再灌注损伤后细胞周期蛋白依赖性激酶抑制因子P21cip1在神经元和星形胶质细胞的表达。方法建立大鼠大脑中动脉阻塞(MCAo)再灌注模型,应用流式细胞术检测各组MCAo再灌注后不同时期神经元和星形胶质细胞中的P21cip1的表达。结果缺血侧皮层区星形胶质细胞和神经元中的P21cip1的表达在再灌注3d、7d、14d后表达下调,与假手术组比较有显著性差异(P<0.05);神经元中的P21cip1的表达和星形胶质细胞中的P21cip1的表达无显著性差异(P>0.05)。结论局灶性脑缺血再灌注损伤后,缺血侧皮层区星形胶质细胞和神经元的p21cip1表达下调。
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| 关 键 词： | 脑缺血 流式细胞术 |
| 修稿时间： | 2007年2月5日 |
THE EXPRESSION OF P21cip1 IN NEURONS AND ASTROCYTES OF RATS AFTER FOCAL CEREBRAL ISCHEMIA |
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| Chen Huaxian,Zhu Zhou,Wang Ping,Zhang Guibin,Yu Zhiyuan,Xie Minjie,Xu Yunlan,Wang Wei.THE EXPRESSION OF P21cip1 IN NEURONS AND ASTROCYTES OF RATS AFTER FOCAL CEREBRAL ISCHEMIA[J].Chinese Journal of Histochemistry and Cytochemistry,2007,16(6):660-663. |
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| Authors: | Chen Huaxian Zhu Zhou Wang Ping Zhang Guibin Yu Zhiyuan Xie Minjie Xu Yunlan Wang Wei |
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| Abstract: | Objective To study the change of expression of P21cip1 in astrocytes and neurons of rats after focal cerebral ischemia.Methods Ischemia was induced by temporary middle cerebral artery occlusion(MCAo).The experimental rats suffered from MCAo were sacrificed on days 1,3,7 and 14 after reperfusion.The brain was taken and the P21cip1of astrocytes and neurons was detected by flow cytometry.Results Compared with that of the controls,the expression of P21cip1 in astrocytes and neurons was remarkably down-regulated on tie ischemic side of cerebral cortex after 3,7 and 14 days of cerebral ischemia/reperfusion(P< 0.05).The P21cip1 expression showed no marked difference between astrocytes and neurons(P> 0.05).Conclusion Astrocytes and neurons have a downregulation of expression of P21cip1after focal cerebral ischemia. |
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| Keywords: | P21cip1 |
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