Stability,purification, and applications of bromelain: A review |
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| Authors: | Letícia Celia de Lencastre Novaes Angela Faustino Jozala André Moreni Lopes Valéria de Carvalho Santos‐Ebinuma Priscila Gava Mazzola Adalberto Pessoa Junior |
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| Institution: | 1. Dept. of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of S?o Paulo, S?o Paulo, Brazil;2. Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), University of Campinas, Campinas, Brazil;3. Dept. of Pharmacy, School of Pharmaceutical Science, Sorocaba University, Sorocaba, Brazil;4. Dept. of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, UNESP—University Estadual Paulista, Araraquara, Brazil |
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| Abstract: | Bromelain is a cysteine protease found in pineapple tissue. Because of its anti‐inflammatory and anti‐cancer activities, as well as its ability to induce apoptotic cell death, bromelain has proved useful in several therapeutic areas. The market for this protease is growing, and several studies exploring various properties of this molecule have been reported. This review aims to compile this data, and summarize the main findings on bromelain in the literature to date. The physicochemical properties and stability of bromelain under different conditions are discussed. Several studies on the purification of bromelain from crude extracts using a wide range of techniques such as liquid–liquid extractions by aqueous two‐phase system, ultrafiltration, precipitation, and chromatography, have been reported. Finally, the various applications of bromelain are presented. This review therefore covers the main properties of bromelain, aiming to provide an up‐to‐date compilation of the data reported on this enzyme. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:5–13, 2016 |
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| Keywords: | bromelain protease anti‐inflammatory downstream processes pineapple purification |
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