Neuropeptide Y,enkephalin and noradrenaline coexist in sympathetic neurons innervating the bovine spleen |
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Authors: | Dr G Fried L Terenius E Brodin S Efendic G Dockray J Fahrenkrug M Goldstein T Hökfelt |
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Institution: | (1) Departments of Physiology, Karolinska Institutet, Stockholm, Sweden;(2) Department of Pharmacology, University of Uppsala, Uppsala, Sweden;(3) Departments of Pharmacology, Karolinska Institutet, Stockholm, Sweden;(4) Department of Endocrinology, Karolinska Hospital, Stockholm, Sweden;(5) Department of Physiology, University of Liverpool, England;(6) Department of Clinical Chemistry, Glostrup and Bispebjerg Hospital, Copenhagen, Denmark;(7) Department of Psychiatry, New York University Medical Center, New York, NY, USA;(8) Departments of Histology, Karolinska Institutet, Stockholm, Sweden;(9) Department of Physiology, Karolinska Institutet, S-104 01 Stockholm, Sweden |
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Abstract: | Summary The subcellular distribution of noradrenaline (NA), neuropeptide Y (NPY), Met and Leu-enkephalin (ENK), substance P (SP), somatostatin (SOM), and vasoactive intestinal polypeptide (VIP) was investigated in homogenates of bovine splenic nerve. The distribution of noradrenergic peptide-containing nerves in the bovine celiac ganglion, splenic nerve and terminal areas in spleen was studied by indirect immunofluorescence histochemistry using antisera to tyrosine hydroxylase (TH), dopamine--hydroxylase (DBH), NPY, enkephalin peptides, SP, SOM, VIP and peptide HI (PHI).After density gradient centrifugation, high levels of NPY and ENK-like immunoreactivity (LI) were found in high-density gradient fractions, coinciding with the main NA peak. SP, SOM and VIP were found in fractions with a lower density, VIP being also enriched in a heavy fraction; the latter three peptides were present in low concentrations.Immunohistochemistry revealed that staining for NPYLI and ENK-LI partly overlapped that for TH and DBH in celiac ganglia, splenic nerve axons and terminal areas of spleen. Almost all principal ganglion cells were TH- and DBH-immunoreactive. Many were also NPY-immunoreactive, whereas a smaller number were ENK-positive. In the celiac ganglion patches of dense SP-positive networks and some VIP/PHI- and ENK-immunoreactive fibers were seen around cell bodies.The results indicate that NPY and ENK are stored with NA in large dense-cored vesicles in unmyelinated axons of bovine splenic nerve. SP, SOM and VIP appear in different organelles in axon populations separate from sympathetic noradrenergic nerves. |
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Keywords: | Neuropeptide Y Enkephalin Noradrenaline Immunohistochemistry Sympathetic neurons Neurotransmitters Spleen Bovine |
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