| MK表达载体的构建及在大肠杆菌中的表达 |
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| 引用本文： | 薛泳涛,黄文晋,石斌,陈镔复,鞠躬.MK表达载体的构建及在大肠杆菌中的表达[J].生物化学与生物物理进展,1998,25(1):56-60. |
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| 作者姓名： | 薛泳涛 黄文晋 石斌 陈镔复 鞠躬 |
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| 作者单位： | 第四军医大学神经科学研究所,西安 710032;第四军医大学神经科学研究所,西安 710032;第四军医大学神经科学研究所,西安 710032;第四军医大学神经科学研究所,西安 710032;第四军医大学神经科学研究所,西安 710032 |
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| 摘 要： | MK是属于肝素结合因子家族的一种多肽,仅在胚胎中期和成年期肾脏表达,在某些肿瘤细胞中也有异常表达.MK能够促进细胞特别是神经细胞的生长和分化,并抑制某些肿瘤细胞的生长.通过RT-PCR从胚胎肾脏中获得了MK成熟肽DNA编码序列,克隆入载体pBV221中,并转入大肠杆菌,建立了重组MK的高表达菌株.
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| 关 键 词： | Midkine(MK)，基因，克隆，表达 |
| 收稿时间： | 1996/12/25 0:00:00 |
| 修稿时间： | 1997/3/17 0:00:00 |
Construction of a High Level Expression Vector of Midkine and Its Expression in E.coli |
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| XUE Yong-tao,HUANG Wei-jin,SHI Bin,CHEN Bin-fu and JU Gong.Construction of a High Level Expression Vector of Midkine and Its Expression in E.coli[J].Progress In Biochemistry and Biophysics,1998,25(1):56-60. |
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| Authors: | XUE Yong-tao HUANG Wei-jin SHI Bin CHEN Bin-fu and JU Gong |
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| Institution: | Institute of Neurosciences, the Fourth Military Medical University, Xi'an 710032, China;Institute of Neurosciences, the Fourth Military Medical University, Xi'an 710032, China;Institute of Neurosciences, the Fourth Military Medical University, Xi'an 710032, China;Institute of Neurosciences, the Fourth Military Medical University, Xi'an 710032, China;Institute of Neurosciences, the Fourth Military Medical University, Xi'an 710032, China |
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| Abstract: | A newly identified gene MK, specifies for a heparin binding factor, is transiently expressed in the midgestation period of embryogenesis and in the early stages of embryonal carcinoma cell differentiation. In late embryos and the adult, MK gene is expressed only in the kidney. MK plays decisive roles not only in growth regulation but also in regulation of cell differentiation. The encoding sequence of mature peptide of MK was obtained by RT-PCR from human fetal kidney and was cloned into pBV221, after transfering into E.coli. A highly expressed vector of MK was constructed. |
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| Keywords: | Midkine gene clone expression |
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