| 一种改进的丝状真菌DNA提取方法 |
| |
| 引用本文： | 张颖慧,魏东盛,邢来君,李明春.一种改进的丝状真菌DNA提取方法[J].微生物学报,2008,35(3):0466-0469. |
| |
| 作者姓名： | 张颖慧 魏东盛 邢来君 李明春 |
| |
| 作者单位： | 南开大学微生物学系 分子微生物学与技术教育部重点实验室 天津 300071;南开大学微生物学系 分子微生物学与技术教育部重点实验室 天津 300071;南开大学微生物学系 分子微生物学与技术教育部重点实验室 天津 300071;南开大学微生物学系 分子微生物学与技术教育部重点实验室 天津 300071 |
| |
| 基金项目： | 天津市科技支撑计划重点项目(已批准, 编号待定) |
| |
| 摘 要： | 以丝状真菌雅致枝霉(Thamnidium elegans)和深黄伞形霉(Umbelopsis isabellina)为材料, 使用优化的CTAB法提取基因组DNA。改进后的方法使用液氮冻融以及玻璃珠振荡的方法代替了传统的液氮研磨, 所需菌体量少, 而得到的基因组DNA比用传统的CTAB法得到的基因组DNA产率高、纯度好、且步骤简单, 适用于一次微量提取多个样品的基因组DNA。这种方法得到的基因组DNA可用于大部分分子生物学基本实验如PCR和DNA的酶切等。
|
| 关 键 词： | DNA提取 CTAB 玻璃珠 液氮冻融 |
A Modified Method for Isolating DNA from Fungus |
| |
| ZHANG Ying-Hui,WEI Dong-Sheng,XING Lai-Jun and LI Ming-Chun.A Modified Method for Isolating DNA from Fungus[J].Acta Microbiologica Sinica,2008,35(3):0466-0469. |
| |
| Authors: | ZHANG Ying-Hui WEI Dong-Sheng XING Lai-Jun and LI Ming-Chun |
| |
| Institution: | Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071;Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071 |
| |
| Abstract: | Genomic DNA of two fungi Thamnidium elegans and Umbelopsis isabellina were extracted with an amended Cetyltrimethyl Ammonium Bromide (CTAB) method. This modified method uses repeated freezing in liquid nitrogen and thawing with combination of shocking with glass beads to replace of the traditional method. Quality and concentration of DNA extracted by the modified methodwere tested. Compared with the traditional method, higher yield and purity of genomic DNA were obtained with less amount ofmycelium. The result indicted that this is a simple and highly efficient method, which is suitable to treat many samples at one time and for basic molecular experiments, such as restriction endonuclease reaction and PCR. |
| |
| Keywords: | Extraction CTAB Glass beads Thawing and freezing test of liquid nitrogen |
|
| 点击此处可从《微生物学报》浏览原始摘要信息 |
| 点击此处可从《微生物学报》下载免费的PDF全文 |
|
