多脂鳞伞多糖提取物对链脲佐菌素诱导糖尿病小鼠抗糖尿病活性

多脂鳞伞Pholiota adiposa是一种珍贵的食药用真菌。本文研究了多脂鳞伞子实体中提取的真菌多糖(PAP)对高脂饮食联合链脲佐菌素诱导的2型糖尿病小鼠的抗糖尿病作用。研究表明,PAP治疗组的空腹血糖水平显著降低,体重增加,并且三酰甘油(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)的水平降低,而超氧化物歧化酶(SOD)、高密度脂蛋白胆固醇(HDL-C)的水平、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)在PAP治疗组中显著增加。模型组的胰腺和肝脏组织表现出显著的病理学变化,PAP治疗组具有改善作用,PAP治疗组胰岛形态和功能显著恢复,此外,蛋白质印迹分析表明,PAP处理后PI3K、AKT、p-AKT、p-IRS1、IRS1、GSK3β和p-GSK3β等蛋白的表达增加,PI3K、p-AKT等信号通路与胰岛素抵抗的减轻相关。综上所述,PAP能降低小鼠空腹血糖水平、调节机体氧化应激性、保护肝脏及胰腺等器官并且能够介导PI3K、p-AKT等信号通路起到预防和治疗糖尿病的作用。

Anti-diabetic effects of fungal polysaccharides extracted from Pholiota adiposa on streptozotocin-induced diabetic mice

Pholiota adiposa is a valuable edible and medicinal fungus. The anti-diabetic effects of the polysaccharides extracted from Pholiota adiposa (PAP) on streptozotocin-induced diabetic ICR male mice were investigated. The results revealed that PAP treatment significantly reducted fasting blood glucose level of mice and increased the body weight. The levels of triacylglycerol (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) were found to be decreased, while the levels of superoxide dismutase (SOD), high-density lipoprotein cholesterol (HDL-C), catalase (CAT), and glutathione peroxidase (GSH-Px) increased. The pancreatic and liver sections of diabetic model control (DC) exhibited notable histopathological changes, but the PAP treatment groups exhibited improvement effect. PAP treatment led to significant restoration of islet morphology and function. The results of Western blotting analysis indicate that the expression of PI3K, p-IRS1, p-GSK3β, and p-AKT increases after PAP treatment. This study comes to the conclusion that PAP exerts an anti-diabetic effect on the diabetic mice.