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| 用高代回交材料筛选与番茄耐冷性相关的RAPD分子标记 | |
| 引用本文： | 赵福宽,杨瑞,林成,高遐虹,程继鸿.用高代回交材料筛选与番茄耐冷性相关的RAPD分子标记[J].生物技术,2004,14(4):8-9. |
| 作者姓名： | 赵福宽 杨瑞 林成 高遐虹 程继鸿 |
| 作者单位： | 1. 北京农学院生物技术系,北京,102206 2. 新疆农业大学园艺学院,新疆,乌鲁木齐,830052 3. 北京农学院植物科技系,北京,102206 |
| 基金项目： | 北京市青年科技新星资助项目 (95381 1 1 0 0 ) |
| 摘 要： | 以番茄冷敏感品系T9801为轮回亲本，以耐冷品系T9806为供体亲本，经7代回交选择获得具有较强耐冷性且具有T9801遗传背景的高代回交株系，从高代回交株系及冷敏感亲本提取DNA，用280个随机引物进行RAPD扩增和多态性分析，筛选到一个与番茄耐冷性相关的RAPD分子标记（OPF14）。 |
| 关 键 词： | 番茄 分子标记 耐冷性 |
| 文章编号： | 1004-311X(2004)04-0008-02 |
| 修稿时间： | 2003年12月11 |
Selection of RAPD Marker Linked to Tomato Cold Tolerance Using Higher Progeny Material of Backcross |
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| ZHAO Fu-kuan,YANG Rui,LIN Cheng,GAO Xia-hong,CHENG Ji-hong.Selection of RAPD Marker Linked to Tomato Cold Tolerance Using Higher Progeny Material of Backcross[J].Biotechnology,2004,14(4):8-9. | |
| Authors: | ZHAO Fu-kuan YANG Rui LIN Cheng GAO Xia-hong CHENG Ji-hong |
| Institution: | ZHAO Fu-kuan~1,YANG Rui~1,LIN Cheng~2,GAO Xia-hong,CHENG Ji-hong~3 |
| Abstract: | Genetic improvement of cold tolerance is an important way to decrease low temperature injury in tomato production.Selection of molecular marker linked to cold tolerance to assist breeding is an effective method in genetic improvement oftomato.Tomato cold sensitive strain (T9801) was used as rotational parent and cold tolerant strain (T9806) was used as supply parent.Higher progeny strain of backcross was obtained through 7 generations backcross selection,which is cold tolerance and has genetic background of T9801.DNA was extracted from higher progeny strain of backcross and cold sensitive parent,280 random primers were used to perform RAPD amplification and polymorphic analysis,a RAPD marker (OPF14) linked to cold tolerance of tomato was identified. |
| Keywords: | tomato molecular marker cold tolerance |
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