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Identification and mapping of a gene conferring resistance to the spot form of net blotch (Pyrenophora teres f maculata) in barley
Authors:K J Williams  A Lichon  P Gianquitto  J M Kretschmer  A Karakousis  S Manning  P Langridge  H Wallwork
Institution:(1) South Australian Research and Development Institute, Urrbrae, South Australia 5064, Australia, AU;(2) ARC Special Research Centre for Basic and Applied Plant Molecular Biology, Department of Plant Science, Waite Campus, University of Adelaide, South Australia 5064, Australia, AU
Abstract: Spot form of net blotch (SFNB) (Pyrenophora teres f maculata) is an economically damaging foliar disease of barley in many of the world’s cereal growing areas. The development of SFNB-resistant cultivars may be accelerated through the use of molecular markers. A screen for SFNB resistance in 96 lines identified four new sources of resistance, including a feed variety, ‘Galleon’, for which a fully mapped doubled haploid population was available. Segregation data indicated SFNB resistance was conferred by a single gene in the ‘Galleon’בHaruna Nijo’ cross, positioned on the long arm of chromosome 7H. This gene is designated Rpt4 and is flanked by the RFLP loci Xpsr117(D) and Xcdo673 at distances of 6.9 cM and 25.9 cM, respectively. The marker Xpsr117(D) was validated using another population segregating for Rpt4, correctly predicting SFNB resistance with more than 90% accuracy. Received: 24 September 1998 / Accepted: 19 December 1998
Keywords:  Hordeum vulgare  Disease resistance  Genetic mapping  RFLP  QTL
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