Biotreatment of nitrate-rich industrial effluent by suspended bacterial growth |
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Authors: | Swati Zala Anuradha Nerurkar Anjana Desai Jayalexmy Ayyer Vivek Akolkar |
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Institution: | (1) Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S, University of Baroda, Baroda, India;(2) Gujarat Narmada Valley Fertilizer Company Ltd., Narmadanagar, Bharuch, India |
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Abstract: | Of the 29 potentially denitrifying organisms isolated from a denitrifying reactor (DNR) of a fertilizer company, two isolates; I-4 and I-5 were recognized as denitrifiers. Under aerobic conditions, with fusel oil as the carbon source, the organisms decreased nitrate from 1200 mg l–1 to 100 mg l–1 in 48 h. Optimal growth conditions for biological removal of nitrate were established in batch culture. The system was scaled up to 4-L and 50-L bioreactors under continuous culture conditions. Up to 95–100% nitrate removal was achieved in the 50-L bioreactor at a COD:NO3–N ratio of 3.45 with a retention time of 48 h. The isolates showed 1.5 fold higher denitrifying activity than reported previously. |
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Keywords: | biotreatment denitrification fertilizer effluent fusel oil |
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