| 抗乙型肝炎病毒表面抗原“y”亚型及“a”组抗原决定簇杂交瘤细胞株的建立与应用 |
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| 引用本文： | 姚仲寅,赵惠玲,陈茜,杨书豪,贾凯,詹美云,张文英,刘崇柏,王风来.抗乙型肝炎病毒表面抗原“y”亚型及“a”组抗原决定簇杂交瘤细胞株的建立与应用[J].病毒学报,1986(3). |
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| 作者姓名： | 姚仲寅 赵惠玲 陈茜 杨书豪 贾凯 詹美云 张文英 刘崇柏 王风来 |
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| 作者单位： | 河南省医学科学研究所
(姚仲寅，赵惠玲，陈茜，杨书豪，贾凯)，中国预防医学科学院病毒学研究所
(詹美云，张文英，刘崇柏)，天津市防病中心(王风来) |
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| 摘 要： | 用部分纯化的HBsAg/adr和ayw亚型免疫Balb/c小鼠的脾细胞,在PEG作用下与Sp2/o骨髓瘤细胞进行融合,经ELISA及RIA法筛选出了分泌抗HBsAg/a(SH 1 D9)、抗HBsAg/y(SG 3 B10)亚型决定簇的杂交瘤细胞系,在组织培养上清液中它们的滴度分别
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| 关 键 词： | 单克隆抗体 乙型肝炎 乙型肝炎病毒表面抗原 “y”亚型 乙肝“a”组抗原决定簇 |
ESTABLISHMENT AND PRELIMINARY APPLICATION OF HYBRIDOMAS SECRETING ANTIBODIES TO HBsAg/a AND HBsAg/Y SUBTYPE ANTIGENIC DETERMINANTS |
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| Yao Zhongyin Zhan Huiling Chen Qian Yang Shuhao Mai Kai Zhan MeiYin Liu Chongbo Zhang Wenying Wang Fenglia Provencial Institute of Medicine of Henan.ESTABLISHMENT AND PRELIMINARY APPLICATION OF HYBRIDOMAS SECRETING ANTIBODIES TO HBsAg/a AND HBsAg/Y SUBTYPE ANTIGENIC DETERMINANTS[J].Chinese Journal of Virology,1986(3). |
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| Authors: | Yao Zhongyin Zhan Huiling Chen Qian Yang Shuhao Mai Kai Zhan MeiYin Liu Chongbo Zhang Wenying Wang Fenglia Provencial Institute of Medicine of Henan |
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| Institution: | Yao Zhongyin Zhan Huiling Chen Qian Yang Shuhao Mai Kai Zhan MeiYin Liu Chongbo Zhang Wenying Wang Fenglia Provencial Institute of Medicine of Henan. Institute of Virology Chinese Academy of Preventive Medicine,The Center of Preventive Disease of Tiangjing Municipalty. |
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| Abstract: | This paper describes splinocytes from Balb/c mice immunized with HBsAg and fused with SP2/0 myloma cells under the action of PEG to yield two hybridomas (SH1D9, SG3B10). Hybrids of SH1D9 and SG3B10 after long-term culture in vitro produced monoclonal antibodies with titers of 1:10000 and 1:8000 respectively by RIA and ELISA, The monoclonal antibody secret- ed by SH1D9 was anti-HBs/a, while that secrected by SG3B10 was anti-HBs/y. All of them belong to the mouse IgG subgroup. Purified McAb/a, y and a+y were used for coating sheep RBC and labelling with 125I to make RPHA-SRBC and RIA reagents respectively. McAb SRBC and 125I-McAb were used to test HBsAg in comparison with PcAb-SRBC and 125I-PcAb. The resulds demonstrated that the monoclonal antibodies havehigh specificity to HBsAg subtype determinant and can be used as a diagnostic reagent to test single antigenic determinants. McAb/y may be useful for the study of HBA/y subtype infection. |
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| Keywords: | Monoclonal antibodies Hepatitis B HBsAg/Y HBsAg/a subtype |
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