Mammalian cell retention devices for stirred perfusion bioreactors |
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Authors: | Steven M Woodside Bruce D Bowen James M Piret |
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Institution: | (1) Biotechnology Laboratory and Department of Chemical and Bio- Resource Engineering, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada |
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Abstract: | Within the spectrum of current applications for cell culture technologies, efficient large-scale mammalian cell production
processes are typically carried out in stirred fed-batch or perfusion bioreactors. The specific aspects of each individual
process that can be considered when determining the method of choice are presented. A major challenge for perfusion reactor
design and operation is the reliability of the cell retention device. Current retention systems include cross-flow membrane
filters, spin-filters, inclined settlers, continuous centrifuges and ultrasonic separators. The relative merits and limitations
of these technologies for cell retention and their suitability for large-scale perfusion are discussed.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | centrifuge cross-flow filter review sedimentation spin-filter ultrasonic cell retention |
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