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Detection of Chlamydia trachomatis by nucleic acid sandwich hybridization

Authors:	Airi Palva Hannele Jousimies-Somer Pekka Saikku Pertti Väänänen Hans Söderlund Marjut Ranki

Institution:	Orion Genetic Engineering Laboratory, Valimotie 7, SF-00380 Helsinki 38, Finland;National Public Health Institute, Mannerheimintie 166, SF-00280 Helsinki 28, Finland;Department of Virology, University of Helsinki, Haartmaninkatu 3, SF-00290 Helsinki 29, Finland;Orion Diagnostica, PL8, SF-02101 Espoo 10, Finland;Recombinant DNA Laboratory, University of Helsinki, Valimotie 7, SF-00380 Helsinki 38, Finland

Abstract:	Abstract Chromosomal DNA fragments from Chlamydia trachomatis serotype L2 were shotgun-cloned into pBR322. A C. trachomatis -specific clone was further subcloned to produce specific DNA reagents for the identification of C. trachomatis by nucleic acid sandwich hybridization. The chosen DNA reagents from serotype L2 also hybridized with all the other chlamydial serotypes tested, but not with the DNA from 41 unrelated organisms. The sensitivity of the sandwich hybridization test was 10⁶ DNA molecules. The applicability of the test for routine diagnostic use was demonstrated by a pilot study in which C. trachomatis was directly detected from genital specimens.

Keywords:	Diagnosis nucleic acid hybridization cloned DNA sexually transmitted disease
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