抗菌肽对种植体周围炎主要致病菌生长抑制作用的研究

目的研究抗菌肽KSL及其衍生物KSL—W对种植体周围炎主要致病菌的体外抑菌效果。方法应用二倍稀释法检测KSL和KSL—W对血链球菌、具梭核杆菌和牙龈卟啉单胞菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）；MTT法检测KSL和KSL—W对成骨样细胞MG-63的细胞毒性。结果KSL和KSL—W对具梭核杆菌的MIC和MBC分别为0．0156mg／mL和0．0313mg／mL，对牙龈卟啉单胞菌的MIC和MBC分别为0．125mg／mL和0．5mg／mL，在0．5mg／mL的浓度范围内对血链球菌没有抑制作用；KSL和KSL-W在0．5mg／mL的浓度范围内没有细胞毒性。结论KSL和KSL—W没有细胞毒性，对具梭核杆菌和牙龈卟啉单胞菌具有抑制作用。

Inhibitory effect of antimicrobial peptides on peri-implantitis pathogens in vitro

Objective To study the inhibitory effect of antimicrobial peptides KSL and its analogues on peri-implantitis pathogens in vitro. Methods The minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of KSL and KSL-W against S. sanguis, F. nucleatum, and P. gingivalis were detected by doubling dilution. Cytotoxicity of KSL and KSL-W were assessed hy the MTT assay. Results The MIC and MBC of KSL and KSL-W to F. nucleatum were 0. 0156 mg/mL and 0. 0313 mg/mL, those to P. gingivalis were 0. 125 mg/mL and 0.5 mg/mL, while those to S. sanguis were not measured under the concentration of 0.5 mg/mL. KSL and KSL-W showed no cytotoxicity under the concentration of 0.5 mg/mL. Conclusion Antimicrobial peptides KSL and KSL-W have no cytotoxieity, but inhibitory effect on F. nucleatum and P. gingivalis.