| 基于主成分分析的双对比光学投影断层成像 |
| |
| 引用本文： | 杨旭伦,张艳婷,关财忠,张章,王陆权,易敏,韩定安,王茗祎,陈勇,曾亚光.基于主成分分析的双对比光学投影断层成像[J].生物化学与生物物理进展,2022,49(4):808-816. |
| |
| 作者姓名： | 杨旭伦 张艳婷 关财忠 张章 王陆权 易敏 韩定安 王茗祎 陈勇 曾亚光 |
| |
| 作者单位： | 1） 佛山科学技术学院机电工程与自动化学院，佛山 528000,2） 佛山科学技术学院物理与光电工程学院，佛山 528000,2） 佛山科学技术学院物理与光电工程学院，佛山 528000,2） 佛山科学技术学院物理与光电工程学院，佛山 528000,1） 佛山科学技术学院机电工程与自动化学院，佛山 528000,2） 佛山科学技术学院物理与光电工程学院，佛山 528000,2） 佛山科学技术学院物理与光电工程学院，佛山 528000,2） 佛山科学技术学院物理与光电工程学院，佛山 528000,1） 佛山科学技术学院机电工程与自动化学院，佛山 528000,2） 佛山科学技术学院物理与光电工程学院，佛山 528000 |
| |
| 基金项目： | 广东省重点领域研究与发展计划（2020B1111040001），国家自然科学基金（61771139，62075042，61605026）和广东省自然科学基金（2017A030313386，2016A020220021）资助项目。 |
| |
| 摘 要： | 目的 本文提出了一种基于主成分分析（PCA）的双对比光学投影断层成像（DC-OPT）方法，以获得活体中血流网络和骨骼的三维可视化。方法 使用主成分分析方法来提取吸收图像和血流图像，原始图像序列的第一主成分用于获取吸收图像；通过计算每个像素的调制深度来获得流动图像。不同投影位置的流动和吸收对比图像被用于三维血流网络和骨骼的同步重建。结果 采用PCA和OPT相结合的方法，通过将动态血流信号和静态背景信号分离，实现了对微生物样本的血流网络和骨骼的三维成像。结论 本文研究的新颖之处在于通过同一光学系统获得了快速、同步、双对比的血流网络和骨骼三维图像。实验结果可用于活体生物的生理发育研究。
|
| 关 键 词： | 光学投影层析成像 主成分分析 双重对比成像 三维血管造影 |
| 收稿时间： | 2021/5/5 0:00:00 |
| 修稿时间： | 2021/7/6 0:00:00 |
Dual-contrast Optical Projection Tomography With Principal Component Analysis |
| |
| YANG Xu-Lun,ZHANG Yan-Ting,GUAN Cai-Zhong,ZHANG Zhang,WANG Lu-Quan,YI Min,HAN Ding-An,WANG Ming-Yi,CHEN Yong and ZENG Ya-Guang.Dual-contrast Optical Projection Tomography With Principal Component Analysis[J].Progress In Biochemistry and Biophysics,2022,49(4):808-816. |
| |
| Authors: | YANG Xu-Lun ZHANG Yan-Ting GUAN Cai-Zhong ZHANG Zhang WANG Lu-Quan YI Min HAN Ding-An WANG Ming-Yi CHEN Yong and ZENG Ya-Guang |
| |
| Institution: | 1) School of Mechatronic Engineering and Automation, Foshan University, Foshan 528000, China,2) School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,2) School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,2) School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,1) School of Mechatronic Engineering and Automation, Foshan University, Foshan 528000, China,2) School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,2) School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,2) School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,1) School of Mechatronic Engineering and Automation, Foshan University, Foshan 528000, China,2) School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China |
| |
| Abstract: | Objective We propose a dual-contrast optical projection tomography (DC-OPT) based on principal component analysis (PCA) to obtain a three-dimensional (3D) visualization of the blood-flow network and skeleton in a live organism.Methods The PCA method was used to extract the absorption and flow contrast images (15 images/projection position). The absorption image was obtained with the use of the first principal component of the raw image sequence. The flow images were obtained based on the computation of the modulation depth of each pixel. The flow and absorption contrast images at different projection positions are combined to achieve a synchronous reconstruction of a 3D image.Results The combination of PCA and OPT is used to realize the 3D imaging of the blood-flow network and skeleton of the biological sample by separating the dynamic blood flow signal and the static background signal of the sample.Conclusion The novelty of our study is attributed to the fast, simultaneous, dual-contrast 3D images of the blood-flow network and skeleton by the same optical system. The experimental result can be applied in physiological development studies in live model organisms. |
| |
| Keywords: | optical projection tomography principal component analysis dual-contrast imaging three-dimensional angiography |
|
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
