Asymmetric Reduction of 4-Oxoisophorone Using Immobilized Thermophilic Bacteria Under Conditions of Controlled Cell Growth |
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Authors: | Koji Sode Nobuaki Hori Yoichi Mikami Isao Karube |
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Institution: |
a Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, Japan
b Central Research Institute, Japan Tobacco Inc., Midori-ku, Yokohama, Japan
c Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan |
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Abstract: | Asymmetric reduction of 2,6,6,-trimethyl-2-cyclohexene-l,4-dione (4-oxoisophrone) to (6R)-2,2,6-trimethyl-1,4-cyclohexane-dione((3R)-dihydro-4-oxoisophorone) was catalysed by immobilized thermophilic bacteria, Thermomonospora curvata JTS 321. Because of leakage of entrapped cells from gel beads during reactions using culture medium, we optimized the medium to allow the microbial conversion under conditions of controlled cell growth. Of the media screened, liver infusion medium was found to be the most suitable and microbial conversion was achieved without cell leakage from the immobilized gels. Immobilized T. curvata cells were repeatedly used for the asymmetric reduction of 4-oxoisophorone, more than 15 times, with an extent of conversion of 50%. |
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Keywords: | Assymetric reduction thermophilic bacteria immobilization growth control 4-oxoisophorone |
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