Micropropagation of 21 species of Mexican cacti by axillary proliferation |
| |
Authors: | Eugenio Pérez Molphe Balch Martha E Pérez Reyes Enrique Villalobos Amador Ernestina Meza Rangel Leticia del Rocío Morones Ruiz Hugo J Lizalde Viramontes |
| |
Institution: | (1) Dpto. de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, 20100 Aguascalientes, Ags, México |
| |
Abstract: | Summary We have developed micropropagation systems for 21 species of Mexican cacti using explants from seedlings germinatedin vitro or shoot segments of juvenile 2–3-yr-old greenhouse plants. The species propagated belong to the generaAstrophytum, Cephalocereus, Coryphantha, Echinocactus, Echinocereus, Echinofossulocactus, Ferocactus, Mammillaria, Nyctocereus, andStenocactus. Multiple shoot formation from areoles was achieved in Murashige and Skoog (MS) medium supplemented with either 1 or 2 mg
N6-benzyladenine (BA) per 1 (4.44 or 8.87 μM) or BA at 1 or 2 mg/l plus naphthaleneacetic acid at 0.1 or 1 mg/l (0.54 or 5.37 μM). The requirements of growth regulators for optimal shoot proliferation, the velocity of the response, and the number of
buds produced by explant were different among the genera and species studied. Rooting of the shoots generatedin vitro was achieved in MS medium supplemented with indoleacetic acid at 0.5–1 mg/l (2.85–5.71 μM) or indolebutyric acid at 0.5–1 mg/l (2.46–4.90 μM). Finally, 70–95% of the rooted plants transferred to potting medium survived. |
| |
Keywords: | Cactaceae tissue culture areole activation plant growth regulators plantlet regeneration |
本文献已被 SpringerLink 等数据库收录! |
|